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Development Genes and Evolution

, Volume 219, Issue 11–12, pp 589–596 | Cite as

Early embryonic development and diapause stage in the band-legged ground cricket Dianemobius nigrofasciatus

  • Naoki Tanigawa
  • Keiji Matsumoto
  • Kouji Yasuyama
  • Hideharu Numata
  • Sakiko ShigaEmail author
Original Article

Abstract

The band-legged ground cricket Dianemobius nigrofasciatus enters diapause at an early embryonic stage when adults are reared under short-day conditions or the eggs are exposed to a low temperature. We examined the morphological features of the embryo during early development and determined the exact stage of entry into diapause. In non-diapause eggs, no periplasmic space was observed in the surface region and a small number of nuclei surrounded by cytoplasm (energids) were found among the yolk granules and lipid droplets 12 h after egg laying (AEL) at 25°C. The energids sparsely but evenly populated the surface region at 40 h AEL, but there were some gaps between these energids. A continuous thin layer of nuclei with cytoplasm had completely covered the egg surface at 56 h AEL, suggesting that the blastoderm is formed between 40 and 56 h AEL. At 72 h AEL, we found a germ band at the posterior pole. Electron microscopy revealed clear cell membranes at 40 h AEL. Staining with rhodamine-dextran dye demonstrated that the cell membrane is formed when the nuclei appear on the egg surface at 12–24 h AEL. These results indicate that cellularization occurs before blastoderm formation. In diapause eggs, neither the embryonic rudiment nor germ band was formed, but a continuous layer of cells covered the egg surface. It is concluded that D. nigrofasciatus enters diapause at the cellular blastoderm.

Keywords

Dianemobius nigrofasciatus Cricket Blastoderm Embryonic diapause Cellularization 

Notes

Acknowledgements

We thank Professor S. Noji and Dr. T. Inoue (Tokushima University, Japan) for their advice on dye injection into the eggs and comments regarding the technical procedures.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Naoki Tanigawa
    • 1
  • Keiji Matsumoto
    • 1
  • Kouji Yasuyama
    • 2
  • Hideharu Numata
    • 1
    • 3
  • Sakiko Shiga
    • 1
    Email author
  1. 1.Department of Biology and Geosciences, Graduate School of ScienceOsaka City UniversityOsakaJapan
  2. 2.Department of BiologyKawasaki Medical SchoolKurashikiJapan
  3. 3.Department of Zoology, Graduate School of ScineceKyoto UniversitySakyoJapan

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