Abstract
Pax genes encode transcription factors that are critical regulators of key developmental processes in evolutionarily diverse animal phyla. Here we report the first isolation of a Pax gene from a crustacean: a Pax group III gene we have termed CdpaxIII that contains highly conserved DNA-binding domains, the paired domain and homeodomain. CdpaxIII is expressed in the embryo, in adult limb muscle during both quiescence and regeneration, and during the distinct process of epimorphic limb regeneration. Interestingly, CdpaxIII is expressed as two distinct alternate transcripts, one of which is novel in lacking a large portion of its paired domain.
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Acknowledgements
The authors wish to thank Meghan Thomas for assistance with preparation of figures, and Brett O’Brien and Julia Wilson for provision of adult yabbies. This work was completed with support from the Centre for Ecosystem Management, Edith Cowan University.
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Communicated by C. Desplan
The sequences reported here have been deposited in the GenBank database [accession numbers AY771613 (CdpaxIII) and AY771614 (CdpaxIIIPAID)]
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White, R.B., Lamey, T.M., Ziman, M. et al. Isolation and expression analysis of a Pax group III gene from the crustacean Cherax destructor. Dev Genes Evol 215, 306–312 (2005). https://doi.org/10.1007/s00427-005-0478-9
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DOI: https://doi.org/10.1007/s00427-005-0478-9