Abstract
The RYK subfamily of receptor tyrosine kinases is characterised by unusual, but highly conserved, amino acid substitutions in the kinase domain. The linotte/derailed gene encodes a Drosophila RYK subfamily member involved in embryonic and adult central nervous system development. Previous studies have shown that the kinase activity of this receptor is not required in vivo for its embryonic function. In this study, we have investigated the role of the cytoplasmic domain and the kinase activity of the linotte/derailed receptor tyrosine kinase in adult brain development. Our results indicate that these domains are not essential for adult brain development but they are required for the proper regulation of the activity of this receptor. This sheds light on a regulatory role for the kinase activity of a RYK subfamily member.
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Acknowledgements
We thank John B. Thomas (UAS-drl and UAS-drlK371A) and the Bloomington Drosophila stock centre (GAL4c155 and UAS-mCD8GFP) for fly stocks. We thank Nicole Lautrédou at the CRIC for valuable help in confocal imaging and Ruth Griffin for helpful discussions and comments on the manuscript. This work was supported by grants from the Association pour la Recherche sur le Cancer (no. 5658), the Fondation pour la Recherche Médicale and the ACI “Biologie du développement et physiologie intégrative” 2003 (BDP0026).
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Edited by C Desplan
Emmanuel Taillebourg and Caroline Moreau-Fauvarque contributed equally to this work
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Taillebourg, E., Moreau-Fauvarque, C., Delaval, K. et al. In vivo evidence for a regulatory role of the kinase activity of the linotte/derailed receptor tyrosine kinase, a Drosophila Ryk ortholog. Dev Genes Evol 215, 158–163 (2005). https://doi.org/10.1007/s00427-004-0457-6
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DOI: https://doi.org/10.1007/s00427-004-0457-6