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her11 is involved in the somitogenesis clock in zebrafish

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Abstract

Somitogenesis requires an intricate process of pre-patterning, which is driven by an oscillator mechanism consisting of the Delta-Notch pathway and hairy- (h) and Enhancer of split- [E(spl)] related genes. With the aim of unravelling the complex mechanism of somite pre-patterning, we have conducted an extensive search for h/E(spl)-related genes in the third release of the Danio rerio genomic sequence. We identified 14 new h/E(spl) genes and analysed them by in situ hybridisation for their potential role in the somitogenesis process. We describe here the functional analysis of one of these genes, which we have named her11. her11 is a paralogue of her1 and, similar to her1, is arranged in a head to head fashion with another her gene, namely the previously described her5. It shares an expression in the midbrain-hindbrain boundary with her5, but is in addition cyclically expressed in patterns overlapping those of her1 and her7 and complementary to those of hey1. Furthermore it is expressed in the anterior half of the most caudally formed somites. We show that Delta-Notch pathway genes and fused somites (fss) are necessary for the control of her11 expression. However, some aspects of the her11 regulation suggest that at least one additional as yet unknown gene of the Delta-Notch cascade is required to explain its expression. Morpholino-oligonucleotide-mediated knockdown of her11 shows that it is involved in the zebrafish somitogenesis clock via an interaction with her1 and her7. We have also studied the role of hey1 by morpholino injection, but could not find a direct function for this gene, suggesting that it reflects the output of the clock rather than being a core component of the mechanism.

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Acknowledgements

We wish to thank Irene Steinfartz and Eva Schetter for excellent technical assistance, Nina Kobs and Bastian Ackermann for fish care. The zebrafish sequence data has been provided freely by the Wellcome Trust Sanger Institute at the Ensembl Genome Browser (http://www.ensembl.org/Danio_rerio/). The work was supported by the Deutsche Forschungsgemeinschaft (SFB 572) and by the Fond der Chemischen Industrie.

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  1. Institut für Genetik, Universität zu Köln, Weyertal 121, 50931, Cologne, Germany

    Dirk Sieger, Diethard Tautz & Martin Gajewski

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  1. Dirk Sieger
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  2. Diethard Tautz
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  3. Martin Gajewski
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Correspondence to Martin Gajewski.

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Edited by B. Herrmann

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Sieger, D., Tautz, D. & Gajewski, M. her11 is involved in the somitogenesis clock in zebrafish. Dev Genes Evol 214, 393–406 (2004). https://doi.org/10.1007/s00427-004-0427-z

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  • DOI: https://doi.org/10.1007/s00427-004-0427-z

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