Abstract
Many members of the Btg/Tob family, which possess anti-proliferation activity, have been identified in mammals but their physiological functions are poorly understood. We have identified a zebrafish tob1 gene, which encodes a peptide of 322 amino acids. The expression pattern of zebrafish tob1 during early embryogenesis is examined by RT-PCR and whole-mount in situ hybridization. The maternal tob1 transcript is abundant in fertilized eggs and blastulas. The expression of tob1 ceases during midgastrulation and early segmentation, when it is expressed and restricted to the notochord and somites. On day 2, expression occurs in the pectoral fin bud but not in other tissues. It will be interesting to investigate whether Tob1 is required for controlling cell proliferation during vertebrate embryogenesis.
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References
Gerber HP, Seipel K, Georgiev O, Hofferer M, Hug M, Rusconi S, Schaffner W (1994) Transcriptional activation modulated by homopolymeric glutamine and proline stretches. Science 263:808–811
Matsuda S, Kawamura-Tsuzuku J, Ohsugi M, Yoshida M, Emi M, Nakamura Y, Onda M, Yoshida Y, Nishiyama A, Yamamoto T (1996) Tob, a novel protein that interacts with p185erbB2, is associated with anti-proliferative activity. Oncogene 12:705–713
Matsuda S, Rouault J, Magaud J, Berthet C (2001) In search of a function for the TIS21/PC3/BTG1/TOB family. FEBS Lett 497:67–72
Sakaguchi T, Kuroiwa A, Takeda H (2001) Expression of zebrafish btg-b, an anti-proliferative cofactor, during early embryogenesis. Mech Dev 104:113–115
Tzachanis D, Freeman GJ, Hirano N, van Puijenbroek AA, Delfs MW, Berezovskaya A, Nadler LM, Boussiotis VA (2001) Tob is a negative regulator of activation that is expressed in anergic and quiescent T cells. Nat Immunol 2:1174–1182
Wienholds E, Schulte-Merker S, Walderich B, Plasterk RH (2002) Target-selected inactivation of the zebrafish rag1 gene. Science 297:99–102
Yoshida Y, Tanaka S, Umemori H, Minowa O, Usui M, Ikematsu N, Hosoda E, Imamura T, Kuno J, Yamashita T, Miyazono K, Noda M, Noda T, Yamamoto T (2000) Negative regulation of BMP/Smad signaling by Tob in osteoblasts. Cell 103:1085–1097
Acknowledgements
We thank Dr. John Postlethwait for chromosomal mapping. This work was supported by NSFC grants (nos. 30025020, 90208002 and 30221003), and TRAPOYT of the MOE.
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Shi, K., Zhang, L. & Meng, A. Cloning and expression analysis of zebrafish tob1 gene. Dev Genes Evol 214, 309–311 (2004). https://doi.org/10.1007/s00427-004-0405-5
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DOI: https://doi.org/10.1007/s00427-004-0405-5