Abstract
The regulation of the Drosophila melanogaster yolk protein genes 1 and 2 have been well characterised. Cis-acting DNA elements and trans-acting factors regulating ovarian fat body and sex-specific expression have been identified. In this paper we have analysed the regulation of yolk protein 3, which is separated from the other two genes on the X-chromosome. We have separated sex-specific control from fat body control in some constructs in transgenic flies. We propose that the organisation of the regulatory elements in yp3 differs from yp1 and yp2 for control of fat body expression and that it closely resembles the regulation of a reporter gene using Musca and Calliphora yp promoter enhancer sequences in transgenic Drosophila.
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Acknowledgements
We would like to thank Sheila Milne for preparation of the manuscript, Kathleen Rothwell for maintenance of fly stocks, members of the group for valuable discussions, and Tim Wood for comments on the manuscript. The pGEX-KG vector was kindly supplied by Ken Burtis. Simone Hutson was supported by a Biotechnology and Biological Sciences Research Council studentship.
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Hutson, S.F., Bownes, M. The regulation of yp3 expression in the Drosophila melanogaster fat body. Dev Genes Evol 213, 1–8 (2003). https://doi.org/10.1007/s00427-002-0286-4
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DOI: https://doi.org/10.1007/s00427-002-0286-4