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Tonoplast inorganic pyrophosphatase in Vicia faba guard cells

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Abstract.

Lysed guard-cell protoplasts of Vicia faba L. exhibited hydrolytic activity characteristic of tonoplast inorganic pyrophosphatase (V-PPase; EC 3.6.1.1). Activity was inhibited by the specific V-PPase inhibitor aminomethylenediphosphonate, stimulated by K+ (K m = 51 mM) and inhibited by Ca2+ (80 nM free Ca2+ was required for 50% inhibition at 0.27 mM free Mg2+). Patch-clamp measurements of electrogenic activity confirmed enzyme localisation at the tonoplast. This is the first report of V-PPase activity in guard cells; its possible involvement in stomatal opening is discussed.

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Authors and Affiliations

  1. Plant Laboratory, Department of Biology, University of York, P.O. Box 373, York YO1 5YW, UK, , , , , , GB

    Catherine P. Darley, Lorna A. Skiera & Dale Sanders

  2. Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK, , , , , , GB

    Frederick D. Northrop & Julia M. Davies

Authors
  1. Catherine P. Darley
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  2. Lorna A. Skiera
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  3. Frederick D. Northrop
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  4. Dale Sanders
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  5. Julia M. Davies
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Received: 12 February 1998 / Accepted: 24 April 1998

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Darley, C., Skiera, L., Northrop, F. et al. Tonoplast inorganic pyrophosphatase in Vicia faba guard cells. Planta 206, 272–277 (1998). https://doi.org/10.1007/s004250050400

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  • DOI: https://doi.org/10.1007/s004250050400

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