A xylosyltransferase that synthesizes β-(1→4)-xylans in wheat (Triticum aestivum L.) seedlings

Abstract.

A particulate preparation from 6-day-old seedlings of wheat (Triticum aestivum L.) was found to contain a xylosyltransferase (XylTase) which incorporated xylose (Xyl) from UDP-xylose into exogenous β-(1→4)-xylooligosaccharides with 2-aminopyridine-derivatized reducing end groups. High-performance liquid chromatographic analysis showed that the chain elongation of pyridylaminated β-(1→4)-xylotriose (Xyl₃-PA) occurred by attachment of a series of one, two, or three xylosyl residues, depending on substrate concentrations and reaction times. Methylation analysis and β-xylosidase digestion of the newly synthesized Xyl₄-PA confirmed that the xylosyl residues were incorporated through β-(1→4)-linkages. The enzyme was maximally active at pH 6.8 and 20 °C, and required Triton X-100, which enhanced activity 5-fold at a concentration of 0.05–2%. Divalent ions, including Mn²⁺ and Mg²⁺, did not affect activity. Enzyme activity increased with increasing polymerization of xylosyl residues of the acceptor substrates: for instance, Xyl₅-PA was almost 7 times as efficient as Xyl₂-PA. The apparent Michaelis constants of the enzyme for Xyl₃-PA and UDP-xylose were 13.5 and 7.9 mM, respectively. The enzyme also catalyzed incorporation of radioactive sugars (Xyl together with a small portion of L-arabinose) from UDP-[¹⁴C]xylose into higher β-(1→4)-xylooligosaccharides (degree of polymerization > 7) with or without (4-O-methyl-)glucuronosyl side chains at activities comparable to those observed for pyridylaminated xylooligosaccharides, and into several heteroxylans but with much lower efficiency. Enzymatic hydrolysis of the product with a β-xylanase degraded it into mainly xylobiose, providing further evidence that the xylosyl residues are incorporated through β-(1→4)-linkages.