Abstract
Main conclusion
The three, by mutagenesis produced genes OsPi21, OsXa5, and OsBADH2, generated novel lines exhibiting desired fragrance and improved resistance.
Abstract
Elite sterile lines are the basis for hybrid rice breeding, and rice quality and disease resistance become the focus of new sterile lines breeding. Since there are few sterile lines with fragrance and high resistance to blast and bacterial blight at the same time in hybrid rice production, we here integrated the simultaneous mutagenesis of three genes, OsPi21, OsXa5, and OsBADH2, into Zhi 5012S, an elite thermo-sensitive genic male sterile (TGMS) variety, using the CRISPR/Cas9 system, thus eventually generated novel sterile lines would exhibit desired popcorn-like fragrance and improved resistance to blast and bacterial blight but without a loss in major agricultural traits such as yield. Collectively, this study develops valuable germplasm resources for the development of two-line hybrid rice with disease resistance, which provides a way to rapid generation of novel TGMS lines with elite traits.
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All data generated or analyzed during this study are included in this published article and its supplementary information files.
Abbreviations
- 2-AP:
-
2-Acetyl-1-pyrroline
- TGMS:
-
Thermo-sensitive genic male sterile
- Xoo :
-
Xanthomonas oryzae pv. oryzae
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Acknowledgements
This work was supported by the Natural Science Foundation of Zhejiang Province (LY24C130004, LY22C135104, and LY23C130002), National Natural Science Foundation of China (31501288), Open Project Program of State Key Laboratory of Rice Biology (20210207), and Central Public-interest Scientific Institution Basal Research Fund (CPSIBRF-CNRRI-202306). We thank Dr. Ruiqing Li (Anhui Agricultural University) for the help with language revision.
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LM and HZ conceived the work. HZ and ML carried out the experiments, performed data analysis; HZ wrote and edited the draft manuscript and prepared the illustrations; LM, HZ, ML, JC and HW discussed the results. LM and HZ formed the final version. All authors have read and approved the final manuscript.
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Zhang, H., Liang, M., Chen, J. et al. Rapid generation of fragrant thermo-sensitive genic male sterile rice with enhanced disease resistance via CRISPR/Cas9. Planta 259, 112 (2024). https://doi.org/10.1007/s00425-024-04392-4
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DOI: https://doi.org/10.1007/s00425-024-04392-4