Abstract
Main conclusion
The loss of TaMYB305 function down-regulated the expression of jasmonic acid synthesis pathway genes, which may disturb the jasmonic acid synthesis, resulting in abnormal pollen development and reduced fertility.
Abstract
The MYB family, as one of the largest transcription factor families found in plants, regulates plant development, especially the development of anthers. Therefore, it is important to identify potential MYB transcription factors associated with pollen development and to study its role in pollen development. Here, the transcripts of an R2R3 MYB gene TaMYB305 from KTM3315A, a thermo-sensitive cytoplasmic male-sterility line with Aegilops kotschyi cytoplasm (K-TCMS) wheat, was isolated. Quantitative real-time PCR (qRT-PCR) and promoter activity analysis revealed that TaMYB305 was primarily expressed in anthers. The TaMYB305 protein was localized in the nucleus, as determined by subcellular localization analysis. Our data demonstrated that silencing of TaMYB305 was related to abnormal development of stamen, including anther indehiscence and pollen abortion in KAM3315A plants. In addition, TaMYB305-silenced plants exhibited alterations in the transcriptional levels of genes involved in the synthesis of jasmonic acid (JA), indicating that TaMYB305 may regulate the expression of genes related to JA synthesis and play an important role during anther and pollen development of KTM3315A. These results provide novel insight into the function and molecular mechanism of R2R3-MYB genes in pollen development.
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Abbreviations
- BSMV:
-
Barley stripe mosaic virus
- JA:
-
Jasmonic acid
- K-TCMS:
-
Thermo-sensitive cytoplasmic male-sterility line with Aegilops kotschyi cytoplasm
- Tns:
-
Trinucleate stage
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Acknowledgements
This research was supported by the National Natural Science Foundation of China (32072060) and Key R&D Program of Shaanxi Province (2022NY-175). We thank the editors and reviewers for their valuable comments on our manuscript.
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LLZ and XYS conceived and designed the experiments. XXW and JCW contributed equally, JCW conducted the experiments, XXW analyzed the datas and wrote the manuscript. ZYL, XYY and XNC participated in data analysis. All authors reviewed the results and approved the final manuscript.
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Wang, X., Wang, J., Liu, Z. et al. The R2R3 MYB gene TaMYB305 positively regulates anther and pollen development in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm. Planta 259, 64 (2024). https://doi.org/10.1007/s00425-024-04339-9
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DOI: https://doi.org/10.1007/s00425-024-04339-9