Abstract
Main conclusion
It was proved for the first time that the miR172e–LbrAP2 module regulated the vegetative growth phase transition in Lilium, which provided a new approach to shorten the juvenile stage of Lilium, improved the reproduction rate, and reduced the propagation cost of Lilium commercial bulbs.
Abstract
Lilium is an ornamental bulb plant that takes at least 3 years to cultivate into commercial seed bulbs under natural conditions. The aim of this study was to shorten the Lilium expansion cycle. In this study, the growth cycle of lily tubers induced by low temperature of 15 °C was significantly shorter than that of tubers grown at a conventional temperature. Quantitative real-time PCR analysis showed that the expression patterns of miR172e and LbrAP2 were negatively correlated. GUS histochemical staining confirmed that miR172e and LbrAP2 in tobacco leaves interacted with each other after co-transformation. The shear sites of miR172e and its target gene, LbrAP2, upon binding, were identified by RLM 5′ RACE analysis. In addition, miR172e and LbrAP2 showed opposite expression patterns after the transformation of Arabidopsis. miR172e overexpression accelerated the transition from juvenile to adult plants, whereas LbrAP2 overexpression inhibited this process, thus indicating that miR172e negatively regulated the target gene LbrAP2. Upregulation of the transcription factor LbrAP2 delayed the phase transition of plants, whereas miR172 inhibited the transcriptional translation of LbrAP2, thereby accelerating the phase transition. Low-temperature treatment of Lilium bulbs can shorten Lilium development, which provides a new approach to accelerating Lilium commercial bulb breeding and reducing breeding costs.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Abbreviations
- AP2:
-
APETALA2
- ERF:
-
ETHYLENE RESPONSIVE FACTOR
- SPL:
-
SQUAMOSA PROMOTER BINDING PROTEIN-LIKE
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Acknowledgements
We are grateful to Dr. Yian Pu of the Institute of Tropical Biotechnology, Chinese Academy of Tropical Agricultural Sciences (ITBB, CATAS) for providing the vector.
Funding
This study was supported by the Beijing Innovation Consortium of Agriculture Research System (project number: BAIC09-2022).
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Conceived and designed the experiments: JF, YW, and KZ. Analyzed the data: JF and WG. Wrote the paper: JF. Revised the paper: JC and JF. All the authors contributed to the article and approved the submitted version.
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Feng, J., Wang, Y., Ge, W. et al. Regulatory mechanism of the miR172e–LbrAP2 module during the vegetative growth phase transition in Lilium. Planta 259, 26 (2024). https://doi.org/10.1007/s00425-023-04308-8
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DOI: https://doi.org/10.1007/s00425-023-04308-8