Abstract
Main conclusion
Different lupin species exhibited varied biomass, P allocation, and physiological responses to P-deprivation. White and yellow lupins had higher carboxylate exudation rates, while blue lupin showed the highest phosphatase activity.
Abstract
White lupin (Lupinus albus) can produce specialized root structures, called cluster roots, which are adapted to low-phosphorus (P) soil. Blue lupin (L. angustifolius) and yellow lupin (L. luteus), which are two close relatives of white lupin, do not produce cluster roots. This study characterized plant responses to nutrient limitation by analyzing biomass accumulation and P distribution, absorption kinetics and root exudation in white, blue, and yellow lupins. Plants were grown in hydroponic culture with (64 µM NaH2PO4) or without P for 31 days. Under P limitation, more biomass was allocated to roots to improve P absorption. Furthermore, the relative growth rate of blue lupin showed the strongest inhibition. Under + P conditions, the plant total-P contents of blue lupin and yellow lupin were higher than that of white lupin. To elucidate the responses of lupins via the perspective of absorption kinetics and secretion analysis, blue and yellow lupins were confirmed to have stronger affinity and absorption capacity for orthophosphate after P-deprivation cultivation, whereas white lupin and yellow lupin had greater ability to secrete organic acids. The exudation of blue lupin had higher acid phosphatase activity. This study elucidated that blue lupin was more sensitive to P-scarcity stress and yellow had the greater tolerance of P-deficient condition than either of the other two lupin species. The three lupin species have evolved different adaptation strategies to cope with P deficiency.
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Data generated and analyzed during the current study are included in this published article and its supplementary information files.
Abbreviations
- ACPase:
-
Acid phosphatase
- DAS:
-
Days after sowing
- LAR:
-
Leaf area ratio
- NAR:
-
Net assimilation rate
- RGR:
-
Relative growth rate
- SLA:
-
Specific leaf area
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This study was supported in part by a Grant-in-Aid for Scientific Research from Japanese Society for the Promotion of Science (21H04717), and in part by a scholarship from the China Scholarship Council under Grant 202106990022.
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RXW, JW, SFN, and CS designed the project and wrote the manuscript. RXW and ZLX conducted the experiment work. All the authors have approved the final manuscript.
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Wang, R., Funayama-Noguchi, S., Xiong, Z. et al. Phosphorus absorption kinetics and exudation strategies of roots developed by three lupin species to tackle P deficiency. Planta 259, 29 (2024). https://doi.org/10.1007/s00425-023-04307-9
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DOI: https://doi.org/10.1007/s00425-023-04307-9