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The Rpf107 gene, a homolog of LOR, is required for the symbiotic nodulation of Robinia pseudoacacia

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Abstract

Main conclusion

Rpf107 is involved in the infection process of rhizobia and the maintenance of symbiotic nitrogen fixation in black locust root nodules.

Abstract

The LURP-one related (LOR) protein family plays a pivotal role in mediating plant defense responses against both biotic and abiotic stresses. However, our understanding of its function in the symbiotic interaction between legumes and rhizobia remains limited. Here, Rpf107, a homolog of LOR, was identified in Robinia pseudoacacia (black locust). The subcellular localization of Rpf107 was analyzed, and its function was investigated using RNA interference (RNAi) and overexpression techniques. The subcellular localization assay revealed that Rpf107 was mainly distributed in the plasma membrane and nucleus. Rpf107 silencing prevented rhizobial infection and hampered plant growth. The number of infected cells in the nitrogen fixation zone of the Rpf107-RNAi nodules was also noticeably lower than that in the control nodules. Notably, Rpf107 silencing resulted in bacteroid degradation and the premature aging of nodules. In contrast, the overexpression of Rpf107 delayed the senescence of nodules and prolonged the nitrogen-fixing ability of nodules. These results demonstrate that Rpf107 was involved in the infection of rhizobia and the maintenance of symbiotic nitrogen fixation in black locust root nodules. The findings reveal that a member of the LOR protein family plays a role in leguminous root nodule symbiosis, which is helpful to clarify the functions of plant LOR protein family and fully understand the molecular mechanisms underlying legume–rhizobium symbiosis.

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Data availability

The Rpf107 gene sequence data generated in this study are available in GenBank of the National Center for Biotechnology Information (NCBI) (https://www.ncbi.nlm.nih.gov/nuccore) under the access numbers: JK974217.

Abbreviations

dpi:

Days post-inoculation

GFP:

Green fluorescent protein

Hpa :

Hyaloperonospora arabidopsidis

ITs:

Infection threads

Lb :

Leghemoglobin gene

LOR:

LURP-one related

LURP1 :

Late upregulated in response to Hpa

PLSCR:

Phospholipid scramblase

R :

Plant disease resistance genes

RNAi:

RNA interference

TEM:

Transmission electron microscopy

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Acknowledgements

This work was supported by National Natural Science Foundation of China (No. 41977052), and Key Research & Development program of Shaanxi Province (No. 2020ZDLNY07-09).

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  1. Yuanli Li and Yuanyuan Wu have contributed equally to this work.

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  1. State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Life Sciences, Northwest A&F University, 3 Taicheng Road, Yangling, 712100, Shaanxi, People’s Republic of China

    Yuanli Li, Yuanyuan Wu, Ziyi Yang, Rui Shi, Lu Zhang, Zhao Feng, Gehong Wei & Minxia Chou

  2. Xiangyang Public Inspection and Testing Center, No.69, Taiziwan Road, Xiangyang, 441000, Hubei Province, People’s Republic of China

    Yuanyuan Wu

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Contributions

YLL analyzed the data and wrote the paper. YLL,YYW, ZYY, RS, LZ, and ZF conducted the experiments and collected data. MXC designed the research and reviewed and edited the manuscript. GHW provided an experimental platform. All authors read and approved the final manuscript.

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Correspondence to Minxia Chou.

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Li, Y., Wu, Y., Yang, Z. et al. The Rpf107 gene, a homolog of LOR, is required for the symbiotic nodulation of Robinia pseudoacacia. Planta 259, 6 (2024). https://doi.org/10.1007/s00425-023-04280-3

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