Abstract
Main conclusion
Ectopic expression of Camellia oleifera Abel. gibberellin 20-oxidase 1 caused a taller phenotype, promoted secondary cell wall deposition, leaf enlargement, and early flowering, and reduced chlorophyll and anthocyanin accumulation and seed enlargement phenotype in Arabidopsis.
Abstract
Plant height and secondary cell wall (SCW) deposition are important plant traits. Gibberellins (GAs) play important roles in regulating plant height and SCWs deposition. Gibberellin 20-oxidase (GA20ox) is an important enzyme involved in GA biosynthesis. In the present study, we identified a GA synthesis gene in Camellia oleifera. The total length of the CoGA20ox1 gene sequence was 1146 bp, encoding 381 amino acids. Transgenic plants with CoGA20ox1 had a taller phenotype; a seed enlargement phenotype; promoted SCWs deposition, leaf enlargement, and early flowering; and reduced chlorophyll and anthocyanin accumulation. Genetic analysis showed that the mutant ga20ox1-3 Arabidopsis partially rescued the phenotype of CoGA20ox1 overexpression plants. The results showed that CoGA20ox1 participates in the growth and development of C. oleifera. The morphological changes in CoGA20ox1 overexpressed plants provide a theoretical basis for further exploration of GA biosynthesis and analysis of the molecular mechanism in C. oleifera.
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Data availability
All data generated or analyzed during this study are included in this published article.
Abbreviations
- GA:
-
Gibberellin
- SCWs:
-
Secondary cell walls
- TPS:
-
Terpene synthase
- ORF:
-
Open reading frame
- POR:
-
Protochlorophyllide oxidoreductase
- CAO:
-
Chlorophyll a oxygenase
- CHS:
-
Chalcone synthase
- CHI:
-
Chalcone isomerase
- F3H:
-
Flavanone 3-hydroxylase
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Acknowledgements
We thank Dr. Xiaoying Zhao of Hunan University for providing ga20ox1-3 seeds.
Funding
This research was funded by the Major Program of Natural Science Foundation of Hunan Province (2021JC0007), Natural Science Foundation of Hunan Province (2023JJ41041), China Postdoctoral Science Foundation (2021M703653), Changsha Natural Science Foundation (kq2202281) and Hunan Forestry Science and Technology Innovation Project (XLK202101-2).
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Wang, Y., Li, J., Guo, P. et al. Ectopic expression of Camellia oleifera Abel. gibberellin 20-oxidase gene increased plant height and promoted secondary cell walls deposition in Arabidopsis. Planta 258, 65 (2023). https://doi.org/10.1007/s00425-023-04222-z
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DOI: https://doi.org/10.1007/s00425-023-04222-z