Abstract
Main conclusion
Arabidopsis GR1 and NTRA function in pollen tube penetrating the stigma into the transmitting tract during pollination.
Abstract
During pollination, recognition between pollen (tube) and stigma mediates the hydration and germination of pollen, as well as the growth of the pollen tube on the stigma. Arabidopsis glutathione reductase 1 (GR1) and NADPH-dependent thioredoxin reductase A (NTRA) are involved in regulating cell redox hemostasis. Both GR1 and NTRA are expressed in pollen, but their roles in pollen germination and the growth of the pollen tube need further investigation. In this study, we performed pollination experiments and found that the Arabidopsis gr1/ + ntra/– and gr1/– ntra/ + double mutation compromised the transmission of male gametophytes. Pollen morphology and viability of the mutants did not show obvious abnormalities. Additionally, the pollen hydration and germination of the double mutants on solid pollen germination medium were comparable to those of the wild type. However, the pollen tubes with gr1 ntra double mutation were unable to penetrate the stigma and enter the transmitting tract when they grew on the surface of the stigma. Our results indicate that GR1 and NTRA play a role in regulating the interaction between the pollen tube and the stigma during pollination.
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The original contributions presented in the study are included in the article. Further inquiries can be directed to the corresponding author.
Abbreviations
- DAPI:
-
4,6-Diamidino-2-phenylindole dihydrochloride
- GFP:
-
Green fluorescent protein
- GR1:
-
Glutathione reductase 1
- NTRA:
-
NADPH-dependent thioredoxin reductase A
- PCP:
-
Pollen coat proteins
- PGM:
-
Pollen germination medium
- RFP:
-
Red fluorescent protein
- ROS:
-
Reactive oxygen species
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This work was supported by the National Natural Science Foundation of China (31970190, 32170361).
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Zhang, M.J., Cui, J.J., Wang, Z.M. et al. GR1 and NTRA involved in pollen tube growth in the stigma of Arabidopsis. Planta 258, 1 (2023). https://doi.org/10.1007/s00425-023-04161-9
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DOI: https://doi.org/10.1007/s00425-023-04161-9