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Relationship between pepper (Capsicum annuum L.) root morphology, inter-root soil bacterial community structure and diversity under water–air intercropping conditions

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Abstract

Main conclusion

The combination of water and gas at an aeration rate of 15 mg/L and irrigation amount of 0.8 Ep significantly promoted the root morphology, inter-root soil bacterial community structure and diversity of pepper, enhanced the structure of molecular symbiotic network, and stimulated the potential ecosystem function.

Abstract

Poor aeration adversely affects the root morphology of pepper (Capsicum annuum L.) and bacterial community. It is critical to understand the effects of water–air interactions on root morphology and bacterial community structure and diversity. A randomized block experiment was conducted under the two aeration rates of dissolved oxygen mass concentrations, including A: 15 mg/L, O: 40 mg/L, and C: non-aeration as control treatment, and two irrigation rates of W1 and W2 (0.8 Ep and 1.0 Ep). The results showed that aerated irrigation had a significant effect on the root morphology of pepper. Compared with treatment CW1, treatment AW1 increased root dry weight, root length, root volume, and root surface area by 13.63%, 11.09%, 59.47%, and 61.67%, respectively (P < 0.05). Aerated irrigation significantly increased the relative abundance of Actinobacteria, Gemmatimonadetes, Alphaproteobacteria, Gemmatimonas, Sphingomonas, and KD4-96 aerobic beneficial bacteria. It decreased the relative abundance of Proteobacteria, Monomycetes, Bacteroidetes, Corynebacterium, Gammaproteobacteria, Anaerolineae, Subgroup_6, MND1, Haliangium, and Thiobacillus. The Pielou_e, Shannon and Simpson indexes of treatment AW1 were significantly higher than treatments OW1 and CW1. The results of the β-diversity of bacterial communities showed that the structure of soil bacterial communities differed significantly among treatments. Actinobacteria was a key phylum affecting root morphology, and AW1 treatment was highly correlated with Actinobacteria. Molecular ecological network analysis showed a relatively high number of bacterial network nodes and more complex relationships among species under the aeration of level 15 mg/L and 0.8 Ep, as well as the emergence of new phylum-level beneficial species: Dependentiae, BRC1, Cyanobacteria, Deinococcus-Thermus, Firmicutes, and Planctomycetes. Therefore, the aeration of 15 mg/L and 0.8 times crop-evaporation coefficient can increase root morphology, inter-root soil bacterial community diversity and bacterial network structure, and enhance potential ecosystem functions in the rhizosphere.

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Data availability

The data reported in this study are contained within the article.

Abbreviations

NMDS:

Non-metric dimensional scaling

RDA:

Redundancy analysis

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Acknowledgements

We want to extend recognition of the support provided financial support by the National Natural Science Foundation of China (No.52079052), the Science and Technology Research Plan in Henan province (212102110032), and the Major Science and Technology Innovation Project in Shandong, Key Research & Development Plan (2019JZZY010710), China.

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Authors and Affiliations

  1. School of Water Conservancy, North China University of Water Resources and Electric Power, Zhengzhou, 450046, China

    Hongjun Lei, Cuicui Jin, Zheyuan Xiao, Jian Chen & Hongwei Pan

  2. College of Land Science and Technology, China Agricultural University, Beijing, 100193, China

    Shah Jahan Leghari

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  1. Hongjun Lei
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  2. Cuicui Jin
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  3. Zheyuan Xiao
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Correspondence to Hongwei Pan.

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Lei, H., Jin, C., Xiao, Z. et al. Relationship between pepper (Capsicum annuum L.) root morphology, inter-root soil bacterial community structure and diversity under water–air intercropping conditions. Planta 257, 98 (2023). https://doi.org/10.1007/s00425-023-04134-y

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