Abstract
Main conclusion
Based on the phenotypic, physiological and transcriptomic analysis, receptor-like kinase HAESA-like 1 was demonstrated to positively affect seed longevity in Arabidopsis.
Abstract
Seed longevity is very important for both genetic resource conservation and crop production. Receptor-like kinases (RLKs) are widely involved in plant growth, development and stress responses. However, the role of most RLKs, especially in seed longevity, is largely unknown. In this study, we report that Arabidopsis HAESA-like 1 (AtHSL1) positively regulated seed longevity. Disruption of HSL1 significantly decreased the germination rate to 50% at 7 days after cold stratification (DAC), compared with that of the wild type (93.5% at 7 DAC), after accelerated aging treatment. Expression of the HSL1 gene in hsl1 basically restored the defective phenotype (86.3%), while HSL1-overexpressing lines (98.3%) displayed slower accelerated aging than WT (93.5%). GUS staining revealed HSL1 was highly expressed universally, especially in young seedlings, mature seeds and embryos of imbibed seeds, and its expression could be induced by accelerated aging. No difference in the dyeing color and area of mucilage were identified between WT and hsl1. The soluble pectin content also was not different, while the adherent pectin content was significantly increased in hsl1. Global transcriptomics revealed that disruption of HSL1 mainly downregulated genes involved in trehalose synthesis, nucleotide sugar metabolism and protection and repair mechanisms. Therefore, an increase in adherent pectin content and downregulation of genes involved in trehalose synthesis may be the main reasons for decreasing seed longevity owing to disruption of HSL1 in Arabidopsis. Our work provides valuable information for understanding the function and mechanism of a receptor-like kinase, AtHSL1, in seed longevity.
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Data availability
The RNA-seq raw data were stored on the NCBI Read Archive database under the accession number PRJNA793755, and will be made publicly accessible after the publication of the manuscript. Other datasets and plant materials generated during the study are available from the corresponding author upon request.
Abbreviations
- DAC:
-
Days after cold stratification
- DEGs:
-
Differentially expressed genes
- GGLT:
-
Golgi nucleotide sugar transporter 3
- HAE:
-
HAESA
- HSL1:
-
HAESA-like 1
- LRR:
-
Leucine-rich repeat
- RHM:
-
Rhamnose synthase
- RLKs:
-
Receptor like kinases
- SSPs:
-
Seed storage proteins
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Acknowledgements
This work was supported by grants from the National Natural Science Foundation of China (No. 32070349 and No. 31870304), and the creative group project of the Rice Industry Technological System of Tianjin (No. ITTRRS20211000-02).
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Chen, D., Guo, H., Chen, S. et al. Receptor-like kinase HAESA-like 1 positively regulates seed longevity in Arabidopsis. Planta 256, 21 (2022). https://doi.org/10.1007/s00425-022-03942-y
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DOI: https://doi.org/10.1007/s00425-022-03942-y