Abstract
Main conclusion
Increased flavonol accumulation and enhanced drought tolerance in A4-rolB-overexpressing plants can be explained by the cooperative action of the SA and ROS signalling pathways.
Abstract
Clarification of function of the A4-rolB plast gene from pRiA4 of Rhizobium rhizogenes will allow a better understanding of the biological principles of the natural transformation process and its use as a tool for plant bioengineering. In the present study, we investigated whether the overexpression of A4-rolB gene could regulate two important processes, flavonoid biosynthesis and drought tolerance. In addition, we investigated some aspects of the possible machinery of the A4-rolB-induced changes in plant physiology, such as crosstalk of the major signalling systems. Based on the data obtained in this work, it can be presumed that constitutive overexpression of A4-rolB leads to the activation of the salicylic acid signalling system. An increase in flavonol accumulation and enhanced drought tolerance can be explained by the cooperative action of SA and ROS pathways.
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Data availability
The data sets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Abbreviations
- DREB:
-
Dehydration-responsive element-binding
- HSF:
-
Heat stress transcription factors
- PFP:
-
P-4-fluoro-DL-phenylalanine
- PR:
-
Pathogenesis-related
- rol :
-
root oncogenic locus
- ROS:
-
Reactive oxygen species
- SA:
-
Salicylic acid
- TF:
-
Transcription factor
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Acknowledgements
Financial support was provided by the Russian Science Foundation, Grant no. 22-24-00082 (Yu. N. Shkryl). The analyses described in this work were performed using equipment from the Instrumental Centre for Biotechnology and Gene Engineering at the Federal Scientific Centre of East Asia Terrestrial Biodiversity of the Far East Branch of the Russian Academy of Sciences.
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Veremeichik, G.N., Shkryl, Y.N., Rusapetova, T.V. et al. Overexpression of the A4-rolB gene from the pRiA4 of Rhizobium rhizogenes modulates hormones homeostasis and leads to an increase of flavonoid accumulation and drought tolerance in Arabidopsis thaliana transgenic plants. Planta 256, 8 (2022). https://doi.org/10.1007/s00425-022-03927-x
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DOI: https://doi.org/10.1007/s00425-022-03927-x