Abstract
Main conclusion
Upregulated expression of RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) encoding a plasma membrane NADPH oxidase is responsible for the lesion-mimic phenotype in detached Arabidopsis leaves with mutation of PHEIDE a OXYGENASE during extended darkness.
Chlorophyll degradation is an indispensable process in leaf senescence, either age-dependent or dark-induced. Besides higher chlorophyll retention, a lesion-mimic phenotype (abbreviated as LMP afterwards) was exhibited in Arabidopsis leaves with mutation of PHEIDE a OXYGENASE (PaO) involved in chlorophyll degradation during dark incubation, but the associated mechanism remains elusive. We found that dark-treated pao leaves showed higher membrane damage and H2O2 accumulation, while scavenging H2O2 by its chemical scavenger diminished LMP. RBOHD which encodes NADPH oxidase was strikingly up-regulated in pao leaves during dark treatment. Chemical inhibition of NADPH oxidase or mutation of RBOHD in pao leaves suppressed LMP. Thus, our study suggests that up-regulated RBOHD transcription is responsible for the formation of LMP in dark-treated pao leaves and there may be a retrograde signaling pathway mediating upregulation of RBOHD which remains to be elucidated.
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Data availability
Sequence information of this article can be found in The Arabidopsis Information Resource (www.arabidopsis.org) or GenBank/EMBL database (www.ebi.ac.uk) under the following accession numbers: NAP (At1g69490), PaO (At3g44880), RBOHD (At5g47910), RBOHF (At1g64060), SOD1 (At1g08830), SOD2 (At4g25100), SOD3 (At3g10920), APX1 (At1g07890), APX3 (At4g35000), APX4 (At4g09010), stromal APX (sAPX, At4g08390), thylakoid APX (tAPX, At1g77490), CAT1 (At1g20630), CAT2 (At4g35090), CAT3 (At1g20620), and UBQ10 (At4g05320).
Abbreviations
- DAB:
-
3,3’-Diaminobenzidine
- DPI:
-
Diphenylene iodonium
- LMP:
-
Lesion-mimic phenotype
- NAC:
-
N-acetyl cysteine
- PaO:
-
Pheide a oxygenase
- RBOHD:
-
Respiratory burst oxidase homolog D
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This work was financially supported by Jiangsu Specially Appointed Professor Program (JSAPP2016) to JY.
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Yu, Y., Zhang, Q., Sun, S. et al. Upregulated expression of RESPIRATORY BURST OXIDASE HOMOLOG D underlies lesion-mimic phenotype in dark-treated Arabidopsis pheide a oxygenase mutant leaves. Planta 255, 110 (2022). https://doi.org/10.1007/s00425-022-03895-2
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DOI: https://doi.org/10.1007/s00425-022-03895-2