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Exogenous ABA promotes aroma biosynthesis of postharvest kiwifruit after low-temperature storage

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Abstract

Main conclusion

Exogenous ABA played a positive role in the accumulation and biosynthesis of aroma components of postharvest kiwifruit after low-temperature storage, especially the esters production during ripening.

Abstract

Low-temperature storage (LTS) generally affects the aroma formation associated with the decrease in aroma quality in kiwifruit. In this work, abscisic acid (ABA) treatment after LTS increased the production of aroma components in postharvest kiwifruit and enhanced the related enzyme activity, especially alcohol acyltransferase (AAT), branched amino acid transaminase (BCAT) and hydroperoxide lyase (HPL). Corresponding to the enzyme activity, the gene expression of AchnAAT, AchnADH, AchnBCAT and AchnHPL was significantly up-regulated by ABA. The principal component analysis further illustrated the differences in aroma components between ABA and the control. The positive correlation of aroma accumulation with the expression levels of AchnPDC and AchnLOX and the enzyme activities of BCAT and pyruvate decarboxylase (PDC) was also revealed by correlation analysis. In addition, the promoter sequences of the key genes involved in aroma biosynthesis contained multiple cis-elements (ABRE and G-box) of ABA-responsive proteins. Combining the transcriptome sequencing data, the promoting role of ABA signaling in the regulation of aroma biosynthesis of postharvest kiwifruit after LTS was discussed. This study would provide a reference for improving aroma quality of postharvest kiwifruit after LTS, as well the molecular mechanism of kiwifruit aroma fading after LTS.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

AAT:

Alcohol acyltransferase

ADH:

Alcohol dehydrogenase

BCAT:

Branched amino acid transaminase

HPL:

Hydroperoxide lyase

LTS:

Low-temperature storage

LOX:

Lipoxygenase

NDGA:

Nordihydroguaiaretic acid

PDC:

Pyruvate decarboxylase

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Acknowledgements

All authors whose names appear in the paper have contributed sufficiently to this work. This work was supported by the General program of the Education Department of Zhejiang Province (Y202044419) and Public Welfare Project of Science and Technology Department of Zhejiang Province (LGN20C150004, LGN19C13003).

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Authors and Affiliations

  1. School of Life Science, Shaoxing University, Shaoxing, 312000, Zhejiang Province, China

    Xueyuan Han, Xiaoyu Wang, Chi Shen, Yiwei Mo, Rungang Tian & Huanyi Yang

  2. College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Agro-Food Processing, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou, 310058, China

    Linchun Mao & Zisheng Luo

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  1. Xueyuan Han
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Correspondence to Huanyi Yang.

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Han, X., Wang, X., Shen, C. et al. Exogenous ABA promotes aroma biosynthesis of postharvest kiwifruit after low-temperature storage. Planta 255, 82 (2022). https://doi.org/10.1007/s00425-022-03855-w

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