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The SNF5-type protein BUSHY regulates seed germination via the gibberellin pathway and is dependent on HUB1 in Arabidopsis

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Abstract

Main conclusion

The SNF5-type protein BUSHY plays a role in the regulation of seed germination via the gibberellin pathway dependent on HUB1 in Arabidopsis thaliana.

Abstract

SWITCH/SUCROSE NONFERMENTING (SWI/SNF) complexes play diverse roles in plant development. Some components have roles in embryo development and seed maturation, however, whether the SNF5-type protein BUSHY (BSH), one of the components, plays a role in Arabidopsis seed related traits is presently unclear. In our study, we show that a loss-of-function mutation in BSH causes increased seed germination in Arabidopsis. BSH transcription was induced by the gibberellin (GA) inhibitor paclobutrazol (PAC) in the seed, and BSH regulates the expression of GA pathway genes, such as Gibberellin 3-Oxidase 1 (GA3OX1), Gibberellic Acid-Stimulated Arabidopsis 4 (GASA4), and GASA6 during seed germination. A genetic analysis showed that seed germination was distinctly improved in the bshga3ox1ga3ox2 triple mutant, indicating that BSH acts partially downstream of GA3OX1 and GA3OX2. Moreover, the regulation of seed germination by BSH in response to PAC is dependent on HUB1. These results provide new insights and clues to understand the mechanisms of phytohormones in the regulation of seed germination.

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Abbreviations

BSH:

SNF5-type protein BUSHY

GA:

Gibberellin

HUB1:

HISTONE MONOUBIQUITINATION1

PAC:

Paclobutrazol

SWI/SNF:

SWITCH/SUCROSE NONFERMENTING complex

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Acknowledgements

We thank Dr. Wim J.J. Soppe very much for his great help on the improvement of the manuscript. This project was supported by the National Natural Science Foundation of China (grant numbers 31171164, 31870305, and 31571257).

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Authors and Affiliations

  1. Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Zhi Wang, Hong Cao, Cun Zhang, Fengying Chen & Yongxiu Liu

  2. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China

    Zhi Wang

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Cun Zhang, Fengying Chen & Yongxiu Liu

  4. The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China

    Yongxiu Liu

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  1. Zhi Wang
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  2. Hong Cao
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Correspondence to Yongxiu Liu.

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Wang, Z., Cao, H., Zhang, C. et al. The SNF5-type protein BUSHY regulates seed germination via the gibberellin pathway and is dependent on HUB1 in Arabidopsis. Planta 255, 34 (2022). https://doi.org/10.1007/s00425-021-03767-1

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