Transcriptome sequencing analysis of sorghum callus with various regeneration capacities

Zhou, Chao; Wang, Sijia; Zhou, Hanlin; Yuan, Zhu; Zhou, Tao; Zhang, Yonghong; Xiang, Sen; Yang, Fang; Shen, Xiangling; Zhang, Dechun

doi:10.1007/s00425-021-03683-4

Original Article
Published: 21 July 2021

Transcriptome sequencing analysis of sorghum callus with various regeneration capacities

Chao Zhou¹^na1,
Sijia Wang¹^na1,
Hanlin Zhou¹,
Zhu Yuan¹,
Tao Zhou¹,
Yonghong Zhang²,
Sen Xiang³,
Fang Yang⁴,
Xiangling Shen¹ &
Dechun Zhang ORCID: orcid.org/0000-0002-6205-2573¹

Planta volume 254, Article number: 33 (2021) Cite this article

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Abstract

Main conclusion

The possible molecular mechanisms regulating sorghum callus regeneration were revealed by RNA-sequencing.

Abstract

Plant callus regeneration has been widely applied in agricultural improvement. Recently, callus regeneration has been successfully applied in the genetic transformation of sorghum by using immature sorghum embryos as explants. However, the mechanism underlying callus regeneration in sorghum is still largely unknown. Here, we describe three types of callus (Callus I–III) with different redifferentiation abilities undergoing distinct induction from immature embryos of the Hiro-1 variety. Compared with nonembryonic Callus III, Callus I produced only some identifiable roots, and embryonic Callus II was sufficient to regenerate whole plants. Genome-wide transcriptome profiles were generated to reveal the underlying mechanisms. The numbers of differentially expressed genes for the three types of callus varied from 5906 to 8029. In accordance with the diverse regeneration abilities observed for different types of callus and leaf tissues, the principal component analysis revealed that the gene expression patterns of Callus I and Callus II were different from those of Callus III and leaves regenerated from Callus II. Notably, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses, pharmacological treatment, and substance content determinations revealed that plant ribosomes, lignin metabolic processes, and metabolism of starch and sucrose were significantly enriched, suggesting that these factors are associated with callus regeneration. These results helped elucidate the molecular regulation of three types of callus with different regeneration abilities in sorghum.

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Acknowledgements

We thank Dr. Hongwei Cai (China Agricultural University) for providing Hiro-1 seeds. The seeds of Hiro-1, a variety of sorghum with small grains from Japan (Han et al. 2015), used in this study were provided by Dr. Hongwei Cai of China Agricultural University (Yuanmingyuan West Road, Beijing, 100193, China). We thank Xiang Peng (China Three Gorges University) for collecting RNA-seq samples. This work was supported by funds from the National Natural Science Foundation of China grants 31900427 (to CZ) and 31371596 (to DZ), the Natural Science Foundation of Hubei Provincial Department of Education (Q20191207) to CZ, the Initial Project for High-Level Personnel of China Three Gorges University to CZ, the Open Project of Hubei Key Laboratory of Wudang Local Chinese Medicine Research (Hubei University of Medicine) (Grant No. WDCM2019009) to CZ, and the Principle Investigator Program (HBMUPI202104) to YZ.

Author information

Chao Zhou and Sijia Wang contributed equally.

Affiliations

Key Laboratory of Three Gorges Regional Plant Genetics and Germplasm Enhancement (CTGU)/Biotechnology Research Center, Yichang Key Laboratory of Omics-Based Breeding for Chinese Medicines, China Three Gorges University, Yichang, 443002, China
Chao Zhou, Sijia Wang, Hanlin Zhou, Zhu Yuan, Tao Zhou, Xiangling Shen & Dechun Zhang
Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan, 442000, China
Yonghong Zhang
School of Information Science and Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China
Sen Xiang
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China
Fang Yang

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Chao Zhou
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Sijia Wang
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Hanlin Zhou
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Zhu Yuan
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Tao Zhou
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Yonghong Zhang
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Sen Xiang
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Fang Yang
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Xiangling Shen
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Dechun Zhang
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Correspondence to Dechun Zhang.

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Zhou, C., Wang, S., Zhou, H. et al. Transcriptome sequencing analysis of sorghum callus with various regeneration capacities. Planta 254, 33 (2021). https://doi.org/10.1007/s00425-021-03683-4

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Received: 28 May 2021
Accepted: 09 July 2021
Published: 21 July 2021
DOI: https://doi.org/10.1007/s00425-021-03683-4

Keywords

Sorghum bicolor
Embryonic callus
Callus regeneration
RNA-seq

Transcriptome sequencing analysis of sorghum callus with various regeneration capacities