Abstract
Main conclusion
4-coumarate-CoA ligase (VIT_02s0109g00250) and copper amine oxidase (VIT_17s0000g09100) played essential roles in contributing to the total soluble solid and total anthocyanin variations induced by bud sport in grape berries.
Abstract
Taste and color, which are important organoleptic qualities of grape berry, undergo rapid and substantial changes during development and ripening. In this study, we used two cultivars ‘Summer Black’ and its bud sport ‘Nantaihutezao’ to explore and identify differentially expressed genes associated with total soluble solid and anthocyanin during developmental stages using RNA-Seq. Overall, substantial differences in expression were observed across berry development between the two cultivars. 5388 genes were detected by weighted gene co-expression network analysis (WGCNA) associated with the total soluble solid (TSS) and anthocyanin contents variations. Several of these genes were significantly enriched in the phenylalanine metabolism pathway; two hub genes 4-coumarate-CoA ligase (VIT_02s0109g00250) and copper amine oxidase (VIT_17s0000g09100) played the most essential roles in relating to the total soluble solid and total anthocyanin variations induced by bud sport through Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and co-expression network analysis. These findings provide insights into the molecular mechanism responsible for the bud sport phenotype.
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Abbreviations
- WGCNA:
-
Weighted gene co-expression network analysis
- TSS:
-
Total soluble solid
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
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This work was supported by the Natural Science Foundation of Jiangsu Province (BK20190899); the doctor for entrepreneurship and innovation program of Jiangsu Province; the Yangzhou City's Green and Golden Phoenix Program.
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Supplementary file1 Suppl. Fig. S1 Real-time quantitative PCR validation of the candidate genes. Genes differentially expressed between ‘Summer Black’ (black bar chart) and its bud sport ‘Nantaihutezao’ (red bar chart). a S5. b S6. c S7. d S8. e S9. f S10 (JPG 233 KB)
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Supplementary file15 Suppl. File S14 Weighted gene co-expression network analysis of the genes was positively correlated with TSS and total anthocyanin (XLSX 23 KB)
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Supplementary file16 Suppl. File S15 Weighted gene co-expression network analysis (WGCNA) of the genes was negatively correlated with TSS and total anthocyanin (XLSX 86 KB)
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Supplementary file17 Suppl. File S16 KEGG pathway enrichment analyses of genes was positively correlated with TSS and total anthocyanin (XLSX 9 KB)
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Supplementary file18 Suppl. File S17 KEGG pathway enrichment analyses of genes was negatively correlated with TSS and total anthocyanin (XLSX 8 KB)
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Supplementary file19 Suppl. File S18 The genes involving in the phenylalanine metabolism pathway were employed to construct gene correlation networks (XLSX 10 KB)
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Leng, F., Ye, Y., Zhu, X. et al. Comparative transcriptomic analysis between ‘Summer Black’ and its bud sport ‘Nantaihutezao’ during developmental stages. Planta 253, 23 (2021). https://doi.org/10.1007/s00425-020-03543-7
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DOI: https://doi.org/10.1007/s00425-020-03543-7