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Identification and characterization of SaeIF1 from the eukaryotic translation factor SUI1 family in cadmium hyperaccumulator Sedum alfredii

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Abstract

Main conclusion

Cadmium-sensitive yeast screening resulted in the isolation of protein translation factor SaeIF1 from the hyperaccumulator Sedum alfredii which has both general and special regulatory roles in controlling cadmium accumulation.

Abstract

The hyperaccumulator of Sedum alfredii has the extraordinary ability to hyperaccumulate cadmium (Cd) in shoots. To investigate its underlying molecular mechanisms of Cd hyperaccumulation, a cDNA library was generated from leaf tissues of S. alfredii. SaeIF1, belonging to the eukaryotic protein translation factor SUI1 family, was identified by screening Cd-sensitive yeast transformants with this library. The full-length cDNA of SaeIF1 has 582 bp and encodes a predicted protein with 120 amino acids. Transient expression assays showed subcellular localization of SaeIF1 in the cytoplasm. SaeIF1 was constitutively and highly expressed in roots and shoots of the hyperaccumulator of S. alfredii, while its transcript levels showed over 100-fold higher expression in the hyperaccumulator of S. alfredii relative to the tissues of a nonhyperaccumulating ecotype of S. alfredii. However, the overexpression of SaeIF1 in yeast cells increased Cd accumulation, but conferred more Cd sensitivity. Transgenic Arabidopsis thaliana expressing SaeIF1 accumulated more Cd in roots and shoots without changes in the ratio of Cd content in shoots and roots, but were more sensitive to Cd stress than wild type. Both special and general roles of SaeIF1 in Cd uptake, transportation, and detoxification are discussed, and might be responsible for the hyperaccumulation characteristics of S. alfredii.

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Abbreviations

Cd:

Cadmium

HP:

Hyperaccumulating ecotype

NHP:

Nonhyperaccumulating ecotype

GFP:

Green fluorescent protein

OV:

Overexpression transgenic plants

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (grant nos. 31770396, and 31300211) and by a National Institutes of Health grant (ES010337). QY was supported by an international graduate fellowship from the China Scholarship Council.

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  1. Qi Yu and Zhong-Chun Zhang contributed equally to this work.

Authors and Affiliations

  1. School of Life Sciences, and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, 430079, Hubei, People’s Republic of China

    Qi Yu, Zhong-Chun Zhang, Miao-Yu Wang & Bao-Sheng Qiu

  2. Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, La Jolla, CA, 92093-0116, USA

    Qi Yu, Alexander Scavo & Julian I. Schroeder

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  1. Qi Yu
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Yu, Q., Zhang, ZC., Wang, MY. et al. Identification and characterization of SaeIF1 from the eukaryotic translation factor SUI1 family in cadmium hyperaccumulator Sedum alfredii. Planta 253, 12 (2021). https://doi.org/10.1007/s00425-020-03539-3

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