Abstract
Main conclusion
AFM, profilometry and SEM measurements on both sides of the Anacardium occidentale L. leaf reveal that ultrastructure presented some singularities due to their different morphologies and roughness.
Abstract
The advanced stereometry and power spectrum density of both sides of the Anacardium occidentale L. leaf were carefully studied. We use three different microscopy techniques such as scanning electron microscopy, profilometry, and atomic force microscopy for a complete description of the leaf surface morphology. The morphology of the adaxial and abaxial sides revealed a surface composed of striated cuticles and stomata cells, respectively. The height parameters obtained by profilometry revealed that the abaxial side was rougher. However, both sides presented similar Gaussian height distribution and asymmetry. The advanced stereometric parameters obtained by the topographic maps of AFM revealed that the two sides have some singularities due to their different morphologies and roughness, but with similar microtextures. However, average PSD spectra showed that adaxial and abaxial sides are dominated by relatively low and high spatial frequencies, showing that the microtextures, unlike what was shown in stereometric parameters, are different. These results revealed that leaves surface morphology under different aspects and scales and the quantitative parameters confirmed the different spatial patterns displayed, which can be of great interest for the study of the biological behavior of plants from their leaves.
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Data availability
The processed data required to reproduce these findings are available by e-mail to the corresponding author: henriquedffilho@yahoo.com.br.
References
Al Afas N, Marron N, Ceulemans R (2006) Clonal variation in stomatal characteristics related to biomass production of 12 poplar (Populus) clones in a short rotation coppice culture. Environ Exp Bot 58:279–286. https://doi.org/10.1016/j.envexpbot.2005.09.003
Arman A, Ţălu Ş, Luna C et al (2015) Micromorphology characterization of copper thin films by AFM and fractal analysis. J Mater Sci Mater Electron 26:9630–9639. https://doi.org/10.1007/s10854-015-3628-5
Baptista A, Gonçalves RV, Bressan J, Pelúzio M, do CG, (2018) Antioxidant and Antimicrobial Activities of Crude Extracts and Fractions of Cashew (Anacardiumoccidentale L.), Cajui (Anacardium microcarpum), and Pequi (Caryocar brasiliense C.): a systematic review. Oxid Med Cell Longev 2018:1–13. https://doi.org/10.1155/2018/3753562
Barcelay YR, Moreira JAG, de Jesus Monteiro Almeida A et al (2020) Nanoscale stereometric evaluation of BiZn0.5Ti0.5O3 thin films grown by RF magnetron sputtering. Mater Lett 1:1. https://doi.org/10.1016/j.matlet.2020.128477
Bashir K, Sohail A, Ali U et al (2020) Foliar micromorphology and its role in identification of the Apocynaceae taxa. Microsc Res Tech 83:755–766. https://doi.org/10.1002/jemt.23466
Blateyron F (2013a) Characterisation of areal surface texture. Springer-Verlag, Berlin Heidelberg, Berlin, Germany
Blateyron F (2013b) The Areal Field Parameters. Characterisation of Areal Surface Texture. Springer Berlin Heidelberg, Berlin, Heidelberg, pp 15–43
Corrêa MM, Melo GAM, Krahl AH, Araújo MGP (2014) Morfoanatomia Foliar de Irlbachia nemorosa (Willd. ex Roem. & Schult.) Merr. (Gentianaceae: Helieae). Biota Amaz 4:5–8. https://doi.org/10.18561/2179-5746/biotaamazonia.v4n2p5-8
Degarmo EP, Black JT, Kohser RA (2019) Materials and Processes in Manufactoring, 13th edn. Wiley, New York
Dong X, Zhu H, Yang X (2015) Characterization of droplet impact and deposit formation on leaf surfaces. Pest Manag Sci 71:302–308. https://doi.org/10.1002/ps.3806
El-Taher AM, El G-NG, Alkahtani J et al (2020) Taxonomic Implication of Integrated Chemical, Morphological, and Anatomical Attributes of Leaves of Eight Apocynaceae Taxa. Diversity 12:334. https://doi.org/10.3390/d12090334
Franco LA, Sinatora A (2015) 3D surface parameters (ISO 25178–2): actual meaning of Spk and its relationship to Vmp. Precis Eng 40:106–111. https://doi.org/10.1016/j.precisioneng.2014.10.011
Gadelmawla ES, Koura MM, Maksoud TMA et al (2002) Roughness parameters. J Mater Process Technol 123:133–145. https://doi.org/10.1016/S0924-0136(02)00060-2
Ifesan B (2013) Antioxidant and Antimicrobial Properties of Selected Plant Leaves. Eur J Med Plants 3:465–473. https://doi.org/10.9734/EJMP/2013/3383
ISO (2012) ISO 25178–2:2012 - Geometrical product specifications (GPS) — Surface texture: Areal — Part 2: Terms, definitions and surface texture parameters. https://www.iso.org/standard/42785.html. Accessed 11 May 2020
Jacobs TDB, Junge T, Pastewka L (2017) Quantitative characterization of surface topography using spectral analysis. Surf Topogr Metrol Prop 5:013001. https://doi.org/10.1088/2051-672X/aa51f8
Jaiswal Y, Naik V, Tatke P et al (2012) Pharmacognostic and preliminary phytochemical investigations on Pterospermum acerifolium wood. Int J Pharma Bio Sci 3:P356–P362
Mabberley DJ (2017) Mabberley’s Plant-book. Cambridge University Press, Cambridge
Martínez JG, Nieto-Carvajal I, Abad J, Colchero J (2012) Nanoscale measurement of the power spectral density of surface roughness: how to solve a difficult experimental challenge. Nanoscale Res Lett 7:174. https://doi.org/10.1186/1556-276X-7-174
Matos RS, Pinto EP, Ramos GQ et al (2020a) Stereometric characterization of kefir microbial films associated with Maytenus rigida extract. Microsc Res Tech. https://doi.org/10.1002/jemt.23532
Matos RS, Ramos GQ, da Fonseca Filho HD, Ţălu Ş (2020b) Advanced micromorphology study of microbial films grown on Kefir loaded with Açaí extract. Micron. https://doi.org/10.1016/j.micron.2020.102912
McHale G, Newton MI, Shirtcliffe NJ (2005) Water-repellent soil and its relationship to granularity, surface roughness and hydrophobicity: a materials science view. Eur J Soil Sci 56:445–452. https://doi.org/10.1111/j.1365-2389.2004.00683.x
Nairn JJ, Forster WA, van Leeuwen RM (2011) Quantification of physical (roughness) and chemical (dielectric constant) leaf surface properties relevant to wettability and adhesion. Pest Manag Sci 67:1562–1570. https://doi.org/10.1002/ps.2213
Nečas D, Klapetek P (2012) Gwyddion: An open-source software for SPM data analysis. Cent Eur J Phys 10:181–188. https://doi.org/10.2478/s11534-011-0096-2
Nezafat NB, Ghoranneviss M, Elahi SM et al (2019) Topographic characterization of canine teeth using atomic force microscopy images in nano-scale. Int Nano Lett 9:311–315. https://doi.org/10.1007/s40089-019-00284-8
Ramos GQ, Cotta EA, da Fonseca Filho HD (2016a) Studies on the ultrastructure in Anacardium occidentale L. leaves from Amazon in northern Brazil by scanning microscopy. Scanning 38:329–335. https://doi.org/10.1002/sca.21274
Ramos GQ, Da Fonseca De AlbuquerqueFerreira MDJLP et al (2016b) Molhabilidade e morfologia da superfície da folha em cajueiro da Amazônia na Região Norte do Brasil. Acta Sci Biol Sci 38:215–220. https://doi.org/10.4025/actascibiolsci.v38i2.30806
Salcedo MOC, Zamora RRM, Carvalho JCT (2016) Study fractal leaf surface of the plant species Copaifera sp. using the Microscope Atomic-Force-AFM. Rev ECIPerú 13:10–16. Available in https://revistaeciperu.com/wpcontent/uploads/2016/08/2revistafisicamarioomarcallasalcedo.pdf.
Samaha MA, Tafreshi HV, Gad-el-Hak M (2012) Superhydrophobic surfaces: from the lotus leaf to the submarine. Comptes Rendus Mécanique 340:18–34. https://doi.org/10.1016/j.crme.2011.11.002
Senthilkumar M, Sahoo NK, Thakur S, Tokas RB (2005) Characterization of microroughness parameters in gadolinium oxide thin films: a study based on extended power spectral density analyses. Appl Surf Sci 252:1608–1619. https://doi.org/10.1016/j.apsusc.2005.02.122
Sobola D, Talu S, Sadovsky P et al (2017) Application of afm measurement and fractal analysis to study the surface of natural optical structures. Adv Electr Electron Eng 15:569–576. https://doi.org/10.15598/aeee.v15i3.2242
Ștefan Ț, Yadav RP, Mittal AK et al (2017) Application of Mie theory and fractal models to determine the optical and surface roughness of Ag–Cu thin films. Opt Quantum Electron 49:256. https://doi.org/10.1007/s11082-017-1079-3
Surf D (2019) MountainsMap Premium 8.0
Zaneta G, Sebastian S, Ştefan T et al (2017) Stereometric parameters of butterfly wings. J Biomimetics Biomater Biomed Eng 31:1–10. https://doi.org/10.4028/www.scientific.net/JBBBE.31.1
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The authors thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the financial support, as well as the use of the infrastructure of the Analytical Center of Universidade Federal do Amazonas (UFAM).
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Communicated by Anastasios Melis.
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Ramos, G.Q., da Costa, Í.C., Maia da Costa, M.E.H. et al. Stereometric analysis of Amazon rainforest Anacardium occidentale L. leaves. Planta 253, 6 (2021). https://doi.org/10.1007/s00425-020-03529-5
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DOI: https://doi.org/10.1007/s00425-020-03529-5