Abstract
Main conclusion
AtKPNB1, an Arabidopsis importin-β protein, was regulated by AtAIRP1 E3 ubiquitin ligase, which intensified the ABA-mediated drought stress response.
Abstract
As an early step in the abscisic acid (ABA)-mediated drought response, the ABA signal is transduced into the nucleus, and thus the nuclear transport system is crucially involved in the drought stress response. AtKPNB1, an importin-β protein, which is a core component of nuclear transport, was previously reported to be a negative factor in the ABA-mediated drought stress response (Luo et al. Luo et al., Plant J 75:377–389, 2013). Here, we report that AtAIPR1, an Arabidopsis RING-type E3 ubiquitin (Ub) ligase, interacted with and ubiquitinated AtKPNB1. A null mutation of AtKPNB1 suppressed the ABA-insensitive germination phenotype of atairp1 mutant seedlings as compared to that of the wild-type plants. Furthermore, the ABA-insensitive stomatal closure and drought-susceptible phenotypes of atairp1 were rescued in atairp1atkpnb1 double mutant progeny, indicating that AtKPNB1 functions downstream of AtAIRP1. These data suggest that AtAIRP1 regulates the ABA-mediated drought response in Arabidopsis via ubiquitination of AtKPNB1.
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Abbreviations
- AIRP1:
-
ABA-INSENSITIVE RING PROTEIN 1
- ATP1/SDIRIP1:
-
AtAIRP2 TARGET PROTEIN 1/SDIR1-INTERACTING PROTEIN 1
- BiFC:
-
Bimolecular fluorescence complementation
- GST:
-
Glutathione S-transferase
- KPNB1:
-
KARYOPHERIN-β 1
- MBP:
-
Maltose-binding protein
- NLS:
-
Nuclear localization signal
- RING:
-
Really Interesting New Gene
- Ub:
-
Ubiquitin
- UPS:
-
Ubiquitin-26S proteasome system
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Acknowledgements
This work was supported by grants from the National Research Foundation (Mid-Career Researcher Program, Project No. 2017R1A2B2006750/2020R1A2B5B02001590 and Basic Science Research Program, Project Number 2018R1A6A1A03025607, Republic of Korea) to W.T.K. S.G.Y was the recipient of a Hyundai Motor Chung Mong-Koo Foundation Scholarship.
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Oh, T.R., Yu, S.G., Yang, H.W. et al. AtKPNB1, an Arabidopsis importin-β protein, is downstream of the RING E3 ubiquitin ligase AtAIRP1 in the ABA-mediated drought stress response. Planta 252, 93 (2020). https://doi.org/10.1007/s00425-020-03500-4
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DOI: https://doi.org/10.1007/s00425-020-03500-4