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Overexpression of wheat transcription factor (TaHsfA6b) provides thermotolerance in barley

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Abstract

Main conclusion

Overexpressing a heat shock factor gene ( TaHsfA6b T) from wheat provides thermotolerance in barley by constitutive expression of heat and other abiotic stress-response genes.

Abstract

Temperature is one of the most crucial abiotic factors defining the yield potential of temperate cereal crops, such as barley. The regulators of heat shock response (HSR), heat stress transcription factors (Hsfs), modulate the transcription level of heat-responsive genes to protect the plants from heat stress. In this study, an Hsf from wheat (TaHsfA6b) is overexpressed in barley for providing thermotolerance. Transgenic barley lines overexpressing TaHsfA6b showed improvement in thermotolerance. The constitutive overexpression of a TaHsfA6b gene upregulated the expression of major heat shock proteins and other abiotic stress-responsive genes. RNA-seq and qRT-PCR analysis confirmed the upregulation of Hsps, chaperonins, DNAJ, LEA protein genes, and genes related to anti-oxidative enzymes in transgenic lines. Excessive generation and accumulation of reactive oxygen species (ROS) occurred in wild-type (WT) plants during heat stress; however, the transgenic lines reflected improved ROS homeostasis mechanisms, showing lesser ROS accumulation under high temperature. No negative phenotypic changes were observed in overexpression lines. These results suggest that TaHsfA6b is a regulator of HSR and its overexpression altered the expression patterns of some main stress-related genes and enhanced the thermotolerance of this cereal crop.

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Abbreviations

HS:

Heat stress

HSR:

Heat stress response

HSF:

Heat stress transcription factor

APX:

Ascorbate peroxidase

CAT:

Catalase

SOD:

Superoxide dismutase

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Acknowledgements

This work was supported by an early career research award (ECR/2016/000084) and core research Grant (CRG/2019/002579) by SERB-DST, Government of India, to HC. AKP, SKM, and PS thank MHRD and RC thanks UGC for providing the research fellowships. PS also thanks the FRQNT, University of Quebec, Trois-Rivières Quebec, Canada for providing the research internship. We also thank Melodie B Plourde and Claire Letanneur for help in Nicotiana infiltration assays.

Funding

This work is supported by research Grants ECR/2016/000084 and CRG/2019/002579 by SERB-DST, Government of India to HC.

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  1. Anuj Kumar Poonia and Sumit Kumar Mishra are contributed equally in this study

Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, 247667, India

    Anuj Kumar Poonia, Sumit Kumar Mishra, Parul Sirohi, Reeku Chaudhary, Meenakshi Kanwar & Harsh Chauhan

  2. Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC, G9A 5H7, Canada

    Parul Sirohi & Hugo Germain

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  1. Anuj Kumar Poonia
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Poonia, A.K., Mishra, S.K., Sirohi, P. et al. Overexpression of wheat transcription factor (TaHsfA6b) provides thermotolerance in barley. Planta 252, 53 (2020). https://doi.org/10.1007/s00425-020-03457-4

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