Abstract
Main conclusion
In Hordeum vulgare, nine differentially expressed novel miRNAs were induced by colchicine. Five novel miRNA in colchicine solution showed the opposite expression patterns as those in water.
Abstract
Colchicine is a commonly used agent for plant chromosome set doubling. MicroRNA-mediated responses to colchicine treatment in plants have not been characterized. Here, we characterized new microRNAs induced by colchicine treatment in Hordeum vulgare using high-throughput sequencing. Our results showed that 39 differentially expressed miRNAs were affected by water treatment, including 34 novel miRNAs and 5 known miRNAs; 42 miRNAs, including 37 novel miRNAs and 5 known miRNAs, were synergistically affected by colchicine and water, and 9 differentially expressed novel miRNAs were induced by colchicine. The novel_mir69, novel_mir57, novel_mir75, novel_mir38, and novel_mir56 in colchicine treatment showed the opposite expression patterns as those in water. By analyzing these 9 differentially expressed novel miRNAs and their targets, we found that novel_mir69, novel_mir56 and novel_mir25 co-target the genes involving the DNA repair pathway. Based on our results, microRNA-target regulation network under colchicine treatment was proposed, which involves actin, cell cycle regulation, cell wall synthesis, and the regulation of oxidative stress. Overall, the results demonstrated the critical role of microRNAs mediated responses to colchicine treatment in plants.
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Abbreviations
- HV0:
-
Barley, untreated
- HV5CK:
-
Barley, 5 h water treatment
- HV5TR:
-
Barley, 5 h colchicine treatment
- NFYA5:
-
Nuclear factor YA family
- TPM:
-
Transcripts per million
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Acknowledgements
This study was financially supported by the National Key Research and Development Program of China (2017YFD0301304) and the international Science and Technology Cooperation Projects of Anhui Province (1704e1002232) to DW, a Research Foundation for Talented Scholars from Anhui Agricultural University and the introduced leading talent research team for Universities in Anhui Province, the Natural Sciences and Engineering Research Council of Canada (RGPIN-2018-05433) to GS.
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Sun, FY., Liu, L., Yu, Y. et al. MicroRNA-mediated responses to colchicine treatment in barley. Planta 251, 44 (2020). https://doi.org/10.1007/s00425-019-03326-9
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DOI: https://doi.org/10.1007/s00425-019-03326-9