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Comparative proteomic analysis of resistant and susceptible potato cultivars during Synchytrium endobioticum infestation

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Abstract

Main conclusion

We report the first comparative study of protein expression profiles in tuber sprouts between Katahdin-derived potato cultivars resistant and susceptible to Synchytrium endobioticum.

Abstract

Synchytrium endobioticum causes wart disease in potato (Solanum tuberosum L.) and is considered as the most important quarantine pathogen in almost all countries where potatoes are grown. We performed a comparative analysis of differentially expressed proteins in the tuber sprouts of potato cultivars differing in resistance to pathotype 1(D1) of S. endobioticum using two-dimensional electrophoresis (2-DE) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) approaches. Bulks prepared from two resistant (Calrose and Humalda) and three susceptible (Sebago, Seneca and Wauseon) potato cultivars were studied. When protein profiles were compared between mock- and S. endobioticum-inoculated sprouts, 35 and 63 protein spots, indicating qualitative or quantitative differences, were detected in the resistant and susceptible cultivars, respectively. In turn, 24 proteins associated with resistance to S. endobioticum were revealed by comparison of the resistant and susceptible bulks. These proteins were changed in a constitutive or induced manner and were grouped into four categories: stress and defence, cell structure, protein turnover, and metabolism. Among the 13 proteins classified into the stress and defence group, seven proteins were related to heat-shock proteins (HSPs)/chaperone factors. In addition, four proteins, S-adenosyl-l-homocysteine hydrolase-like, superoxide dismutase [Mn], inactive patatin-3-Kuras 1 and patatin-15, were induced in the resistant bulk; whereas two proteins, patatin-01 and nucleoredoxin 1, showed significant differences in expression between the S. endobioticum-inoculated resistant and susceptible bulks. The detection of such a large number of S. endobioticum-mediated proteins representing the HSP70, HSP60 and HSP20 families suggests their significant role in restricting wart disease in potato tubers.

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Acknowledgements

The research was supported by Polish Ministry of Science and Higher Education (project 1-3-00-1-01) and National Science Center in Poland (project No. 2013/11/B/NZ9/01959).

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Authors and Affiliations

  1. Plant Breeding and Acclimatization Institute-National, Research Institute, Młochów Research Center, Platanowa 19, 05-831, Młochów, Poland

    Katarzyna Szajko, Jarosław Plich, Dorota Sołtys-Kalina & Waldemar Marczewski

  2. Plant Breeding and Acclimatization Institute-National Research Institute, Radzików, 05-870, Błonie, Poland

    Jarosław Przetakiewicz

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  1. Katarzyna Szajko
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  2. Jarosław Plich
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  3. Jarosław Przetakiewicz
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Correspondence to Waldemar Marczewski.

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Szajko, K., Plich, J., Przetakiewicz, J. et al. Comparative proteomic analysis of resistant and susceptible potato cultivars during Synchytrium endobioticum infestation. Planta 251, 4 (2020). https://doi.org/10.1007/s00425-019-03306-z

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