Abstract
Main conclusion
Specific combinations of physiological and molecular parameters associated with N and S remobilization measured at the onset of flowering were predictive of final crop performances in oilseed rape.
Abstract
Oilseed rape (Brassica napus L.) is a high nitrogen (N) and sulphur (S) demanding crop. Nitrogen- and S-remobilization processes allow N and S requirements to reproductive organs to be satisfied when natural uptake is reduced, thus ensuring high yield and seed quality. The quantification of physiological and molecular indicators of early N and S remobilization could be used as management tools to correct N and S fertilization. However, the major limit of this corrective strategy is to ensure the correlation between final performances-related variables and early measured parameters. In our study, four genotypes of winter oilseed rape (OSR) were grown until seed maturity under four nutritional modalities combining high and/or low N and S supplies. Plant final performances, i.e., seed production, N- and S-harvest indexes, seed N and S use efficiencies, and early parameters related to N- or S-remobilization processes, i.e., photosynthetic leaf area, N and S leaf concentrations, leaf soluble protein and leaf sulphate concentrations, and leaf RuBisCO abundance at flowering, were measured. We demonstrated that contrasting final performances existed according to the N and S supplies. An optimal N:S ratio supply could explain the treatment-specific crop performances, thus justifying N and S concurrent managements. Specific combinations of early measured plant parameters could be used to predict final performances irrespective of the nutritional supply and the genotype. This work demonstrates the potential of physiological and molecular indicators measured at flowering to reflect the functioning of N- and S-compound remobilization and to predict yield and quality penalties. However, because the predictive models are N and S independent, instant N and S leaf analyses are required to further adjust the adequate fertilization. This study is a proof of a concept which opens prospects regarding instant diagnostic tools in the context of N and S mineral fertilization management.
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Abbreviations
- °Cd:
-
Degree day
- HI:
-
Harvest index
- HN/HS:
-
High nitrogen/high sulphur
- LN/LS:
-
Low nitrogen/low sulphur
- LSU/SSU:
-
Large/small subunits of RuBisCO
- NHI/SHI:
-
Nitrogen/sulphur harvest index
- OSR:
-
Oilseed rape
- PrS:
-
Projected surface
- SP:
-
Soluble protein
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Acknowledgements
The authors thank the BrACySol (Center of biological resources in INRA Rennes, France) for providing the seeds. They are grateful to C. Salon, C. Bernard, K. Palavioux, C. Jeudy, F. Zenk, D. Girondé, and N. El-Miyad for their helpful advice and technical assistance in the 4PMI. They are also very grateful to J. Pichon, M. Bodereau, J. Bonnefoy, and M. Hodel for plant sample preparation and B. Andrieu for his helpful comments on the experimental design and on the manuscript. They also thank PLATIN’, the Plateau d’Isotopie de Normandie ICORE facility for performing the elemental analyses. They eventually thank S. Lavenant-Lemauviel for her helpful advice to perform the correlation and principal component analyses. Genergy and Rapsodyn projects funded by National Research Agency (ANR) and the French program “Investments for the Future”.
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Akmouche, Y., Cheneby, J., Lamboeuf, M. et al. Do nitrogen- and sulphur-remobilization-related parameters measured at the onset of the reproductive stage provide early indicators to adjust N and S fertilization in oilseed rape (Brassica napus L.) grown under N- and/or S-limiting supplies?. Planta 250, 2047–2062 (2019). https://doi.org/10.1007/s00425-019-03284-2
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DOI: https://doi.org/10.1007/s00425-019-03284-2