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Planta

, Volume 250, Issue 1, pp 145–162 | Cite as

Transcriptome and hormone analyses provide insights into hormonal regulation in strawberry ripening

  • Tingting GuEmail author
  • Shufen Jia
  • Xiaorong Huang
  • Lei Wang
  • Weimin Fu
  • Guotao Huo
  • Lijun Gan
  • Jing Ding
  • Yi LiEmail author
Original Article

Abstract

Main conclusion

The possible molecular mechanisms regulating strawberry fruit ripening were revealed by plant hormone quantification, exogenous hormone application, and RNA-sequencing.

Fruit ripening involves a complex interplay among plant hormones. Strawberry is a model for studies on non-climacteric fruit ripening. However, the knowledge on how plant hormones are involved in strawberry ripening is still limited. To understand hormonal actions in the ripening process, we performed genome-wide transcriptome and hormonal analysis for the five major hormones (abscisic acid and catabolites, auxins, cytokinins, gibberellins, and ethylene) in achenes and receptacles (flesh) at different ripening stages of the woodland strawberry Fragaria vesca. Our results demonstrate that the pre-turning stage (a stage with white flesh and red achenes defined in this study) is the transition stage from immature to ripe fruits. The combinatorial analyses of hormone content, transcriptome data, and exogenous hormone treatment indicate that auxin is synthesized predominantly in achenes, while abscisic acid (ABA), bioactive free base cytokinins, gibberellins, and ethylene are mainly produced in receptacles. Furthermore, gibberellin may delay ripening, while ethylene and cytokinin are likely involved at later stages of the ripening process. Our results also provide additional evidence that ABA promotes ripening, while auxin delays it. Although our hormone analysis demonstrates that the total auxin in receptacles remains relatively low and unchanged during ripening, our experimental evidence further indicates that ABA likely enhances expression of the endoplasmic reticulum-localized auxin efflux carrier PIN-LIKES, which may subsequently reduce the auxin level in nucleus. This study provides a global picture for hormonal regulation of non-climacteric strawberry fruit ripening and also evidence for a possible mechanism of ABA and auxin interaction in the ripening process.

Keywords

Fragaria Fruit ripening Hormone cross-talks Plant hormone quantification Transcriptome 

Abbreviations

ABAGE

ABA glucose ester

BW

Big white

CK

Cytokinin

DEG

Differentially expressed gene

DPA

Days after anthesis

FPKM

Fragments per kilobase per million

iP

Isopentenyladenine

NDGA

Nordihydroguaiaretic acid

Pre-T

Pre-turning

SW

Small white

Notes

Acknowledgements

We thank Dr. Yang Yuan and Decai Liu for technical support for the bioinformatic analysis. This work was supported by the National Natural Science Foundation of China [31672123 to T.G., 31471860 to J.D.] and the Fundamental Research Funds for the Central Universities [KYZ201605 to T.G.].

Supplementary material

425_2019_3155_MOESM1_ESM.doc (222 kb)
Supplementary material 1 (DOC 221 kb)
425_2019_3155_MOESM2_ESM.pptx (6 mb)
Supplementary material 2 (PPTX 6103 kb)
425_2019_3155_MOESM3_ESM.xlsx (112 kb)
Supplementary material 3 (XLSX 112 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Genetics and Germplasm Enhancement and College of HorticultureNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Laboratory of Plant hormone, College of Life SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China
  3. 3.Department of Plant Science and Landscape ArchitectureUniversity of ConnecticutStorrsUSA

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