, Volume 250, Issue 1, pp 129–143 | Cite as

A novel QTL QTrl.saw-2D.2 associated with the total root length identified by linkage and association analyses in wheat (Triticum aestivum L.)

  • Xingwei Zheng
  • Xiaojie Wen
  • Ling Qiao
  • Jiajia Zhao
  • Xiaojun Zhang
  • Xin Li
  • Shuwei Zhang
  • Zujun Yang
  • Zhijian Chang
  • Jianli ChenEmail author
  • Jun ZhengEmail author
Original Article


Main conclusion

In wheat, a QTL QTrl.saw-2D.2 associated with the total root length was identified, presumably containing genes closely related to root development.

A mapping population of 184 recombinant inbred lines derived from the cross SY95-71 × CH7034 was used to map QTL for seedling root characteristics in hydroponic culture (HC) and in soil-filled pot (SP) methods. Four traits, including maximum root length (MRL), root number (RN), total length (TRL), and root diameter (RD) were measured and used in QTL analyses. A total of 33 QTL for the four root traits were detected, 17 QTLs for TRL, six for RN, seven for MRL, and three for RD. Seven QTL were detected in both HC and SP methods, which explained 7–18% phenotypic variation. One QTL QTrl.saw-2D.2 detected in both HC and SP methods was also validated in another population comprised of 215 diverse lines. This QTL is a novel QTL that explained the highest phenotypic variation 18% in all QTL identified in the present study. Based on candidate gene and comparative genomics analyses, the QTL QTrl.saw-2D.2 may contain genes closely related to root development in wheat (Triticum aestivum L.). The two candidate genes were proposed to explore in future studies.


Drought tolerance coefficient Quantitative trait locus Stay green Total root length Yield components 



Dryland conditions


Drought tolerance coefficient


Hydroponic culture


Kernel number per spike


Maximum root length


Root diameter


Recombinant inbred line


Root number


Spike length


Spikelet number per spike


Pots containing soil


Thousand kernel weight


Total root length


Well-watered condition



This work was funded by National Key Research and Development Program of China (2017YFD0100600), Agricultural Science and Technology Project (YCX2018413, 17yzgc010), Natural Science Foundation of Shanxi Province (2016011001, 201703D211007). We thank Dr. Robert A McIntosh (University of Sydney) for help with manuscript improvement.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Xingwei Zheng
    • 1
  • Xiaojie Wen
    • 2
  • Ling Qiao
    • 1
  • Jiajia Zhao
    • 1
  • Xiaojun Zhang
    • 3
  • Xin Li
    • 3
  • Shuwei Zhang
    • 3
  • Zujun Yang
    • 3
  • Zhijian Chang
    • 3
  • Jianli Chen
    • 4
    Email author
  • Jun Zheng
    • 1
    Email author
  1. 1.Institute of Wheat ResearchShanxi Academy of Agricultural SciencesLinfenChina
  2. 2.Biotechnology Research InstituteChinese Academy of Agricultural SciencesBeijingChina
  3. 3.The Shanxi Province Key Laboratory of Crop Genetics and Gene Improvement, Institute of Crop ScienceShanxi Academy of Agricultural SciencesTaiyuanChina
  4. 4.Department of Plant SciencesUniversity of IdahoAberdeenUSA

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