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Planta

, Volume 249, Issue 6, pp 1963–1975 | Cite as

Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance

  • Lili You
  • Qiping Song
  • Yuyong Wu
  • Shengchun Li
  • Chunmei Jiang
  • Ling Chang
  • Xinghong YangEmail author
  • Jiang ZhangEmail author
Original Article
  • 232 Downloads

Abstract

Main conclusion

Plastid genome engineering is an effective method to generate drought-resistant potato plants accumulating glycine betaine in plastids.

Glycine betaine (GB) plays an important role under abiotic stress, and its accumulation in chloroplasts is more effective on stress tolerance than that in cytosol of transgenic plants. Here, we report that the codA gene from Arthrobacter globiformis, which encoded choline oxidase to catalyze the conversion of choline to GB, was successfully introduced into potato (Solanum tuberosum) plastid genome by plastid genetic engineering. Two independent plastid-transformed lines were isolated and confirmed as homoplasmic via Southern-blot analysis, in which the mRNA level of codA was much higher in leaves than in tubers. GB accumulated in similar levels in both leaves and tubers of codA-transplastomic potato plants (referred to as PC plants). The GB content was moderately increased in PC plants, and compartmentation of GB in plastids conferred considerably higher tolerance to drought stress compared to wild-type (WT) plants. Higher levels of relative water content and chlorophyll content under drought stress were detected in the leaves of PC plants compared to WT plants. Moreover, PC plants presented a significantly higher photosynthetic performance as well as antioxidant enzyme activities during drought stress. These results suggested that biosynthesis of GB by chloroplast engineering was an effective method to increase drought tolerance.

Keywords

Compartmentation Drought stress Genetic improvement Glycine betaine Plastid transformation Potato 

Abbreviations

GB

Glycine betaine

COD

Choline oxidase

RWC

Relative water contents

MDA

Malondialdehyde

qP

Photochemical quenching

NPQ

Non-photochemical quenching

ETR II

Electron transport rate of PSII

ROS

Reactive oxygen species

SOD

Superoxide dismutase

POD

Peroxidase

CAT

Catalase

APX

Ascorbate peroxidase

Notes

Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (31572071), the Science and Technology Department of Hubei Province of China (2016CFA052) and the Recruitment Program of Global Experts (China) to J. Z. Authors are also grateful to Dr. Mingyu Wu (Hubei University, Wuhan) for helping with soil moisture measurement.

Supplementary material

425_2019_3132_MOESM1_ESM.docx (11 kb)
Supplementary material 1 (DOCX 11 kb)
425_2019_3132_MOESM2_ESM.docx (1.3 mb)
Supplementary material 2 (DOCX 1300 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019
corrected ​publication 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Biocatalysis and Enzyme Engineering, College of Life SciencesHubei UniversityWuhanChina
  2. 2.College of Life Science, State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop BiologyShandong Agricultural UniversityTaianChina

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