, Volume 249, Issue 5, pp 1627–1643 | Cite as

Apple AP2/EREBP transcription factor MdSHINE2 confers drought resistance by regulating wax biosynthesis

  • Ya-Li Zhang
  • Chun-Ling Zhang
  • Gui-Luan Wang
  • Yong-Xu Wang
  • Chen-Hui Qi
  • Chun-Xiang You
  • Yuan-Yuan LiEmail author
  • Yu-Jin HaoEmail author
Original Article


Main conclusion

This study showed that AP2/EREBP transcription factor MdSHINE2 functioned in mediating cuticular permeability, sensitivity to abscisic acid (ABA), and drought resistance by regulating wax biosynthesis.

Plant cuticular wax plays crucial roles in protecting plants from environmental stresses, particularly drought stress. Many enzymes and transcription factors involved in wax biosynthesis have been identified in plant species. In this study, we identified an AP2/EREBP transcription factor, MdSHINE2 from apple, which is a homolog of AtSHINE2 in Arabidopsis. MdSHINE2 was constitutively expressed at different levels in various apple tissues, and the transcription level of MdSHINE2 was induced substantially by abiotic stress and hormone treatments. MdSHINE2-overexpressing Arabidopsis exhibited great change in cuticular wax crystal numbers and morphology and wax composition of leaves and stems. Moreover, MdSHINE2 heavily influenced cuticular permeability, sensitivity to abscisic acid, and drought resistance.


Apple AP2/EREBP ABA sensitivity Cuticular permeability Crystal morphology Drought resistance Wax load 














We would like to thank Prof. Takaya Moriguchi of the National Institute of Fruit Tree Science, Japan, for ‘Orin’ apple calli. We would also like to thank EditorBar Language Editing Company for providing linguistic assistance during the preparation of this manuscript. This study was financially supported by the National Natural Science Foundation of China (31772275) and the Natural Science Fund for Excellent Young Scholars of Shandong Province (ZR2018JL014).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Key Laboratory of Crop Biology, College of Horticulture Science and EngineeringShandong Agricultural UniversityTai-anChina

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