Engineering cyanobacteria for production of terpenoids
- 436 Downloads
This review summarizes recent advances in cyanobacterial terpenoid production. The challenges and opportunities of improving terpenoid production by cyanobacteria are discussed.
Terpenoids are a diverse group of natural products with a variety of commercial applications. With recent advances in synthetic biology and metabolic engineering, microbial terpenoid synthesis is being viewed as a feasible approach for industrial production. Among different microbial hosts, cyanobacteria have the potential of sustainable production of terpenoids using light and CO2. Terpene synthases and the precursor pathways have been expressed in cyanobacteria for enhanced production of various terpene hydrocarbons, including isoprene, limonene, β-phellandrene, and farnesene. However, the productivities need to be further improved for commercial production. Many barriers remain to be overcome in order to efficiently convert CO2 to terpenoids. In this review, we will summarize recent efforts on photosynthetic production of terpenoids and discuss the challenges and opportunities of engineering cyanobacteria for terpenoid bioproduction.
KeywordsTerpenoid Cyanobacteria Metabolic engineering MEP pathway MVA pathway
1-Deoxy-d-xylulose 5-phosphate synthase
Isopentenyl diphosphate isomerase
Funding to support this work was provided by the Office of Science (BER), U. S. Department of Energy, to HBP. PCL was supported by a fellowship from the McDonnell International Scholars Academy at Washington University.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Abernathy MH, Yu J, Ma F, Liberton M, Ungerer J, Hollinshead WD, Gopalakrishnan S, He L, Maranas CD, Pakrasi HB, Allen DK, Tang YJ (2017) Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis. Biotechnol Biofuels 10:273. https://doi.org/10.1186/s13068-017-0958-y CrossRefGoogle Scholar
- Brenner MP, Bildsten L, Dyson F, Fortson N, Garwin R, Grober R, Hemley R, Hwa T, Joyce G, Katz J (2006) Engineering microorganisms for energy production. DTIC DocumentGoogle Scholar
- Colby SM, Alonso WR, Katahira EJ, McGarvey DJ, Croteau R (1993) 4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase. J Biol Chem 268(31):23016–23024Google Scholar
- Toth TN, Chukhutsina V, Domonkos I, Knoppova J, Komenda J, Kis M, Lenart Z, Garab G, Kovacs L, Gombos Z, van Amerongen H (2015) Carotenoids are essential for the assembly of cyanobacterial photosynthetic complexes. Biochem Biophys Acta 1847(10):1153–1165. https://doi.org/10.1016/j.bbabio.2015.05.020 Google Scholar
- van der Woude AD, Angermayr SA, Puthan Veetil V, Osnato A, Hellingwerf KJ (2014) Carbon sink removal: increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant. J Biotechnol 184:100–102. https://doi.org/10.1016/j.jbiotec.2014.04.029 CrossRefGoogle Scholar
- Zhou C, Li Z, Wiberley-Bradford AE, Weise SE, Sharkey TD (2013) Isopentenyl diphosphate and dimethylallyl diphosphate/isopentenyl diphosphate ratio measured with recombinant isopentenyl diphosphate isomerase and isoprene synthase. Anal Biochem 440(2):130–136. https://doi.org/10.1016/j.ab.2013.05.028 CrossRefGoogle Scholar