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Planta

, Volume 248, Issue 1, pp 69–87 | Cite as

Proteomic analysis of melatonin-mediated osmotic tolerance by improving energy metabolism and autophagy in wheat (Triticum aestivum L.)

  • Guibin Cui
  • Fengli Sun
  • Xinmei Gao
  • Kunliang Xie
  • Chao Zhang
  • Shudong Liu
  • Yajun Xi
Original Article

Abstract

Main conclusion

Melatonin-mediated osmotic tolerance was attributed to increased antioxidant capacity, energy metabolism, osmoregulation and autophagy in wheat (Triticum aestivum L.).

Melatonin is known to play multiple roles in plant abiotic stress tolerance. However, its role in wheat has been rarely investigated. In this study, 25% polyethylene glycol 6000 (PEG 6000) was used to simulate osmotic stress, and wheat seeds and seedlings were treated with different concentrations of melatonin under PEG stress. Isobaric tag for relative and absolute quantification (iTRAQ)-based proteomic techniques were used to identify the differentially accumulated proteins from melatonin-treated and non-treated seedlings. Seeding priming with melatonin significantly increased the germination rate, coleoptile length, and primary root number of wheat under PEG stress, as well as the fresh weight, dry weight, and water content of wheat seedlings. Under PEG stress, melatonin significantly improved reactive oxygen species homeostasis, as revealed by lower H2O2 and O 2 · content; and the expression of antioxidant enzymes at the transcription and translation levels was increased. Melatonin maintained seedling growth by improving photosynthetic rates and instantaneous and intrinsic water use efficiencies, as well as carbon fixation and starch synthesis at the protein level. Melatonin treatment significantly affected the expression of glycolytic proteins, including fructose-1,6-bisphosphate aldolase, hexokinase, glyceraldehyde-3-phosphate dehydrogenase, and enolase, and remarkably increased the expression of the nicotinamide adenine dinucleotide transporter and nicotinamide adenine dinucleotide binding protein, thereby indirectly modulating electron transport in the respiratory chain. This indicated that melatonin improved energy production in PEG-stressed seedlings. Further, melatonin played a regulatory role in autophagy, protease expression, and ubiquitin-mediated protein degradation by significantly upregulating rab-related protein, fused signal recognition particle receptor, aspartyl protease, serine protease, ubiquitin-fold modifier 1, and ubiquitin at the mRNA or protein level. These findings suggested that melatonin might activate a metabolic cascade related to autophagy under PEG stress in wheat seedlings.

Keywords

Cytophagy Drought stress Glycolysis iTRAQ N-Acetyl-5-methoxytryptamine Polyethylene glycol (PEG) Seed priming 

Abbreviations

iTRAQ

Isobaric tag for relative and absolute quantification

DP

Differentially accumulated proteins

Notes

Acknowledgements

We are grateful for the funding support for this study from The National Key Basic Research Program, China (2017YFD0100706), Protection and Utilization of Germplasm Resources of Shaanxi Province, China (20171010000004), Transgenic Major Projects of China (2014ZX0800203B), and Key Science and Technology Program of Shaanxi Province, China (2013K02-01-02).

Supplementary material

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Supplementary material 1 (DOCX 16 kb)
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Supplementary material 4 (XLSX 19 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Guibin Cui
    • 1
  • Fengli Sun
    • 1
  • Xinmei Gao
    • 1
  • Kunliang Xie
    • 1
  • Chao Zhang
    • 1
  • Shudong Liu
    • 1
  • Yajun Xi
    • 1
  1. 1.College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Wheat Biology and Genetic Improvement on Northwestern China, Ministry of AgricultureNorthwest A&F UniversityYanglingChina

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