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Planta

, Volume 248, Issue 1, pp 49–68 | Cite as

Counteractive mechanism (s) of salicylic acid in response to lead toxicity in Brassica juncea (L.) Czern. cv. Varuna

  • Ashish Agnihotri
  • Praveen Gupta
  • Anuj Dwivedi
  • Chandra Shekhar Seth
Original Article
  • 232 Downloads

Abstract

Main conclusion

Salicylic acid alleviates lead toxicity in Brassica juncea (L.) by promoting growth under non-stress and activating stress-defense mechanism (s) under lead stress conditions. It also boosts the ascorbate–glutathione cycle and thus helps in minimizing oxidative and DNA damage.

Brassica juncea plants were exposed to different concentrations (0, 500, 1000 and 2000 mg kg−1) of lead (Pb) and subsequently sprayed with 0.5 mM of salicylic acid (SA) to check for morphological and leaf gas exchange parameters like transpiration rate (E), stomatal conductance (GH2O), net photosynthetic rate (A) and maximum quantum yield of PS II (Fv/Fm). Leaf epidermis by scanning electron microscopy (SEM), enzymatic and non-enzymatic components of ascorbate–glutathione (AsA–GSH) cycle, DNA damage by comet assay, lipid peroxidation and endogenous SA quantification by HPLC were analyzed. Lead accumulation in root, shoot and its sub-cellular distribution ratio (SDR) and localization was also determined using atomic absorption spectroscopy (AAS) and rhodizonate-dye staining method, respectively. Results revealed that notable amount of Pb was accumulated in root and shoot in dose-dependent manner which significantly (P ≤ 0.05) posed the toxicity on the majority of morphological parameters, structural integrity of epidermal and guard cells, photosynthetic pigments, malondialdehyde (MDA) and H2O2 content. Notable decrease in leaf gas exchange parameters, Fv/Fm, poor performance of AsA–GSH cycle and striking amount of DNA damage, was found as well. However, SA revoked Pb toxicity to a great extent by promoting growth, chlorophyll content, improving the A, Fv/Fm, boosting the overall performance of AsA–GSH cycle and by lessening the DNA damage.

Keywords

Ascorbate–glutathione cycle Brassica juncea Chlorophyll fluorescence DNA damage Lead Oxidative stress Photosynthetic rate Salicylic acid Sub-cellular distribution ratio 

Abbreviations

A

Net photosynthetic rate

AsA

Reduced ascorbate

E

Transpiration rate

GH2O

Stomatal conductance

GSH

Oxidized glutathione

HM

Heavy metal

SA

Salicylic acid

Notes

Acknowledgements

The present work is supported by Research and Development Grant of University of Delhi, India, and DU-DST Purse Grant of Department of Science and Technology, New Delhi, India. Mr. Ashish Agnihotri is deeply indebted to Council of Scientific and Industrial Research (CSIR), New Delhi, India, for awarding the Senior Research Fellowship (SRF). The technical staffs of Central Instrumentation Facility, Department of Botany, are also acknowledged for their support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ashish Agnihotri
    • 1
  • Praveen Gupta
    • 1
  • Anuj Dwivedi
    • 1
    • 2
  • Chandra Shekhar Seth
    • 1
  1. 1.Department of BotanyUniversity of DelhiDelhiIndia
  2. 2.National Institute of Plant Genome ResearchNew DelhiIndia

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