O-acetylserine(thio)lyase (OAS-TL) molecular expression in Pancratium maritimum L. (Amaryllidaceae) under salt stress
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Different levels of salt stress affected the OAS-TL expression levels in Pancratium maritimum organs (bulb, leaf and root). A detailed method has been described for the identification of the conserved domain of the OAS-TL cDNA in sea daffodil given the scarce data available for the Amaryllidaceae family.
Pancratium maritimum or sea daffodil (Amaryllidaceae) is a bulbous geophyte growing on coastal sands. In this study, we investigated the involvement of cysteine synthesis for salt tolerance through the expression of the enzyme O-acetylserine(thio)lyase (OAS-TL) during the stress response to NaCl treatments in P. maritimum. Quantitative real-time PCR was used in different organs (bulb, leaf and root).
KeywordsPsammophilous Real-time PCR Salt stress Sand dune Sea daffodil
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We gratefully acknowledge the Nando Peretti Foundation for funding the study (Project 2012-83). For field sampling of Pancratium maritimum, we would like to thank Dr. Rossella Muoio and Giancarlo Sibilio from Botanical Garden of Naples (University of Naples Federico II, Italy). Finally, a special thanks to Prof. Luciano Gaudio (University of Naples Federico II) for giving us the possibility to believe in ourselves.
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