Planta

, Volume 247, Issue 3, pp 773–777 | Cite as

O-acetylserine(thio)lyase (OAS-TL) molecular expression in Pancratium maritimum L. (Amaryllidaceae) under salt stress

  • Olga De Castro
  • Michele Innangi
  • Bruno Menale
  • Simona Carfagna
Short Communication
  • 88 Downloads

Abstract

Main conclusion

Different levels of salt stress affected the OAS-TL expression levels in Pancratium maritimum organs (bulb, leaf and root). A detailed method has been described for the identification of the conserved domain of the OAS-TL cDNA in sea daffodil given the scarce data available for the Amaryllidaceae family.

Pancratium maritimum or sea daffodil (Amaryllidaceae) is a bulbous geophyte growing on coastal sands. In this study, we investigated the involvement of cysteine synthesis for salt tolerance through the expression of the enzyme O-acetylserine(thio)lyase (OAS-TL) during the stress response to NaCl treatments in P. maritimum. Quantitative real-time PCR was used in different organs (bulb, leaf and root).

Keywords

Psammophilous Real-time PCR Salt stress Sand dune Sea daffodil 

Abbreviations

BLAST

Basic Local Alignment Search Tool

OAS-TL

O-acetylserine(thio)lyase

Notes

Acknowledgements

We gratefully acknowledge the Nando Peretti Foundation for funding the study (Project 2012-83). For field sampling of Pancratium maritimum, we would like to thank Dr. Rossella Muoio and Giancarlo Sibilio from Botanical Garden of Naples (University of Naples Federico II, Italy). Finally, a special thanks to Prof. Luciano Gaudio (University of Naples Federico II) for giving us the possibility to believe in ourselves.

Supplementary material

425_2018_2855_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiologyUniversity of Naples Federico IINaplesItaly
  2. 2.Department of Environmental, Biological, Pharmaceutical Sciences and TechnologiesUniversity of Campania “Luigi Vanvitelli”CasertaItaly

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