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Planta

, Volume 249, Issue 5, pp 1285–1299 | Cite as

Specialized lysophosphatidic acid acyltransferases contribute to unusual fatty acid accumulation in exotic Euphorbiaceae seed oils

  • Jay Shockey
  • Ida Lager
  • Sten Stymne
  • Hari Kiran Kotapati
  • Jennifer Sheffield
  • Catherine Mason
  • Philip D. BatesEmail author
Original Article
  • 297 Downloads

Abstract

Main conclusion

In vivo and in vitro analyses of Euphorbiaceae species’ triacylglycerol assembly enzymes substrate selectivity are consistent with the co-evolution of seed-specific unusual fatty acid production and suggest that many of these genes will be useful for biotechnological production of designer oils.

Many exotic Euphorbiaceae species, including tung tree (Vernicia fordii), castor bean (Ricinus communis), Bernardia pulchella, and Euphorbia lagascae, accumulate unusual fatty acids in their seed oils, many of which have valuable properties for the chemical industry. However, various adverse plant characteristics including low seed yields, production of toxic compounds, limited growth range, and poor resistance to abiotic stresses have limited full agronomic exploitation of these plants. Biotechnological production of these unusual fatty acids (UFA) in high yielding non-food oil crops would provide new robust sources for these valuable bio-chemicals. Previous research has shown that expression of the primary UFA biosynthetic gene alone is not enough for high-level accumulation in transgenic seed oils; other genes must be included to drive selective UFA incorporation into oils. Here, we use a series of in planta molecular genetic studies and in vitro biochemical measurements to demonstrate that lysophosphatidic acid acyltransferases from two Euphorbiaceae species have high selectivity for incorporation of their respective unusual fatty acids into the phosphatidic acid intermediate of oil biosynthesis. These results are consistent with the hypothesis that unusual fatty acid accumulation arose in part via co-evolution of multiple oil biosynthesis and assembly enzymes that cooperate to enhance selective fatty acid incorporation into seed oils over that of the common fatty acids found in membrane lipids.

Keywords

Diacylglycerol acyltransferase Eleostearic acid Ricinoleic acid Lysophosphatidic acid acyltransferase Triacylglycerol 

Abbreviations

DGAT

Diacylglycerol acyltransferase

ESA

Eleostearic acid

FADX

Tung tree fatty acid conjugase X

FAH

Castor fatty acid hydroxylase

FAME

Fatty acid methyl ester

FID

Flame ionization detection

GPAT

Glycerol-3-phosphate acyltransferase

HFA

Hydroxy fatty acids

HPLC

High-performance liquid chromatography

GC

Gas chromatography

LPA

Lysophosphatidic acid

LPAT

Lysophosphatidic acid acyltransferase

PCR

Polymerase chain reaction

PDAT

Phospholipid: diacylglycerol acyltransferase

PDCT

Phosphatidylcholine:diacylglycerol cholinephosphotransferase

TLC

Thin layer chromatography

UFA

Unusual fatty acid

Notes

Acknowledgements

The authors would like to thank Ms. Tien Thuy Vuong for technical assistance. This work was supported by the U.S. Department of Agriculture, Agricultural Research Service Current Research Information System project number 6054-41000-102-00D (to JS and CM) and the National Science Foundation (Directorate for Biological Sciences, Division of Molecular and Cellular Bioscience, award #1613923, to PDB and JS).

Supplementary material

425_2018_3086_MOESM_ESM.pdf (614 kb)
Supplementary material 1 (PDF 613 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.United States Department of Agriculture, Agricultural Research Service, Southern Regional Research CenterNew OrleansUSA
  2. 2.Department of Plant BreedingSwedish University of Agricultural SciencesAlnarpSweden
  3. 3.Department of Chemistry and BiochemistryUniversity of Southern MississippiHattiesburgUSA
  4. 4.Institute of Biological ChemistryWashington State UniversityPullmanUSA

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