Characterization of an acetohydroxy acid synthase mutant conferring tolerance to imidazolinone herbicides in rice (Oryza sativa)
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The acetohydroxy acid synthase S627N mutation confers herbicide tolerance in rice, and the rice variety containing this mutation produces good yields. This variety is commercially viable at Shanghai and Jiangsu regions in China.
Weedy rice is a type of rice that produces lower yields and poorer quality grains than cultivated rice. It plagues commercial rice fields in many countries. One strategy to control its proliferation is to develop rice varieties that are tolerant to specific herbicides. Acetohydroxy acid synthase (AHAS) mutations have been found to confer herbicide tolerance to rice. Here, we identified a single mutation (S627N) in AHAS from an indica rice variety that conferred tolerance against imidazolinone herbicides, including imazethapyr and imazamox. A japonica rice variety (JD164) was developed to obtain herbicide tolerance by introducing the mutated indica ahas gene. Imidazolinone application was sufficient to efficiently control weedy rice in the JD164 field. Although the imazethapyr treatment caused dwarfing in the JD164 plants, it did not significantly reduce yields. To determine whether the decrease of the ahas mRNA expression caused the dwarfism of JD164 after imazethapyr application, we detected the ahas mRNA level in plants. The abundance of the ahas mRNA in JD164 increased after imidazolinone application, thus excluding the mRNA expression level as a possible cause of dwarfism. Activity assays showed that the mutated AHAS was tolerant to imidazolinone but the catalytic efficiency of the mutated AHAS decreased in its presence. Moreover, the activity of the mutated AHAS decreased more in the presence of imazethapyr than in the presence of imazamox. We observed no difference in the AHAS secondary structures, but homology modeling suggested that the S627N mutation enabled the substrate to access the active site channel in AHAS, resulting in imidazolinone tolerance. Our work combined herbicides with a rice variety to control weedy rice and showed the mechanism of herbicide tolerance in this rice variety.
KeywordsS627N mutation JD164 Yields Activities Molecular structures
The research was funded by Grants to Jun Fang from Natural Science Foundation of Shanghai (No. 15ZR1436500), Foundation of Shanghai Agricultural Talents (No. HNQZ2016-1-1), Key Project of Agriculture Science and Technology of Shanghai (No. HNKGZ2015-6-1-3) and SAAS Excellent Research Team (No. NKC2017A05).
- Akhgari H, Kaviani B (2011) Assessment of direct seeded and transplanting methods of rice cultivars in the northern part of Iran. Afr J Agric Res 6(3):6492–6498Google Scholar
- Biasini M, Bienert S, Waterhouse A, Arnold K, Studer G, Schmidt T, Kiefer F, Gallo Cassarino T, Bertoni M, Bordoli L, Schwede T (2014) SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information. Nucl Acids Res 42(Web Server issue):252–258. https://doi.org/10.1093/nar/gku340 CrossRefGoogle Scholar
- Chen X, Qiang S, Yang J, Zhang B, Zhang Z, Song X, Dai W (2015) Hierarchical clustering and indica-japonica classification: uncover mutual spread and indica-japonica differentiation for weedy rice in Jiangsu province. Chin J Rice Sci 1:82–90Google Scholar
- Gutteridge S, Thompson ME, Ort O, Shaner DL, Stidham M, Singh B, Tan S, Johnson TC, Mann RK, Schmitzer PR, Gast RE, deBoer GJ, Yoshimura T, Hanai R (2012) Acetohydroxyacid synthase inhibitors (AHAS/ALS). In: Krämer W, Schirmer U, Jeschke P and Witschel M (eds) Modern Crop Protection Compounds, 2nd edn. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, pp 29–162CrossRefGoogle Scholar
- Guttieri MJ, Eberlein CV, Mallory-Smith CA, Hoffman DL (1992) DNA-sequence variation in domain a of the acetolactate synthase genes of herbicide-resistant and herbicide-susceptible weed biotypes. Weed Sci 40:670–676Google Scholar
- Huang X, Yang S, Gong J, Zhao Q, Feng Q, Zhan Q, Zhao Y, Li W, Cheng B, Xia J, Chen N, Huang T, Zhang L, Fan D, Chen J, Zhou C, Yiqi L, Weng Q, Han B (2016) Genomic architecture of heterosis for yield traits in rice. Nature 537:329–633Google Scholar
- Islam MK, Islam MS, Biswas JK, Siyoung L, Alam I, Mooryong H (2014) Screening of rice varieties for direct seeding method. Aust J Crop Sci 8(4):536–542Google Scholar
- Kanapeckas KL, Vigueira CC, Ortiz A, Gettler KA, Burgos NR, Fischer AJ, Lawton-Rauh AL (2016) Escape to ferality: the endoferal origin of weedy rice from crop rice through de-domestication. PLoS One 11(9):e0162676. https://doi.org/10.1371/journal.pone.0162676 CrossRefPubMedPubMedCentralGoogle Scholar
- Li T, Liu B, Chen CY, Yang B (2016) TALEN-mediated homologous recombination produces site-directed dna base change and herbicide-resistant rice. J Genet Genom 43:291–305Google Scholar
- Liang Y, Guo S, Yin L (2014) Comparative study on morphological differences between weedy rice and cultivate rice in Shanghai area. J Shanghai Norm Univ (Nat Sci) 43(1):87–97Google Scholar
- Mallory-Smith CA, Thill DC, Dial MJ (1990) Identification of sulfonylurea herbicide-resistant prickly lettuce (Lactuca serriola). Weed Technol 4(1):163–168Google Scholar
- Nelson KA, Renner KA, Penner D (1998) Weed control in soybean (Glycine max) with imazamox and imazethapyr. Weed Sci 46(5):587–594Google Scholar
- Normile D (2014) China pulls plug on genetically modified rice and corn. Science. http://www.sciencemag.org/news/2014/08/china-pulls-plug-genetically-modified-rice-and-corn. Accessed 20 Aug 2014
- Ott KH, Kwagh JG, Stockton GW, Sidorov V, Kakefuda G (1996) Rational molecular design and genetic engineering of herbicide resistant crops by structure modeling and site-directed mutagenesis of acetohydroxyacid synthase. J Mol Biol 263(2):359–368. https://doi.org/10.1006/jmbi.1996.0580 CrossRefPubMedGoogle Scholar
- Wang Z, Rong J, B-r Lu (2015) Occurence and damage of weedy rice and its threats to rice production in China. Weed Sci (China) 33(1):1–9Google Scholar
- Zhang L, Chen H, Li Y, Li Y, Wang S, Su J, Liu X, Chen D, Chen X (2014) Evaluation of the agronomic performance of atrazine-tolerant transgenic japonica rice parental lines for utilization in hybrid seed production. PLoS One 9(9):e108569. https://doi.org/10.1371/journal.pone.0108569 CrossRefPubMedPubMedCentralGoogle Scholar