Gene structure, expression pattern and interaction of Nuclear Factor-Y family in castor bean (Ricinus communis)
- 436 Downloads
Nuclear Factor-Y transcription factors, which function in regulating seed development (including storage reservoir accumulation) and responding to abiotic stresses, were identified and characterized in castor bean.
Nuclear Factor-Y (NF-Y) transcription factors in plants contain three subunits (NF-YA, NF-YB and NF-YC), and function as a heterodimer or heterotrimer complex in regulating plant growth, development and response to stresses. Castor bean (Ricinus communis, Euphorbiaceae) one of the most economically important non-edible oilseed crops, able to grow in diverse soil conditions and displays high tolerance to abiotic stresses. Due to increasing demands for its seed oils, it is necessary to elucidate the molecular mechanism underlying the regulation of growth and development. Based on the available genome data, we identified 25 RcNF-Y members including six RcNF-YAs, 12 RcNF-YBs and seven RcNF-YCs, and characterized their gene structures. Yeast two-hybrid assays confirmed the protein–protein interactions among three subunits. Using transcriptomic data from different tissues, we found that six members were highly or specifically expressed in endosperms (in particular, two LEC1-type members RcNF-YB2 and RcNF-YB12), implying their involvement in regulating seed development and storage reservoir accumulation. Further, we investigated the expression changes of RcNF-Y members in two-week-old seedlings under drought, cold, hot and salt stresses. We found that the expression levels of 20 RcNF-Y members tested were changed and three RcNF-Y members might function in response to abiotic stresses. This study is the first reported on genomic characterization of NF-Y transcription factors in the family Euphorbiaceae. Our results provide the basis for improved understanding of how NF-Y genes function in the regulation of seed development and responses to abiotic stresses in both castor bean and other plants in this family.
KeywordsAbiotic stress Castor bean Expression profiles NF-Y transcription factor Protein interaction
Leafy Cotyledon 1
Yeast two hybrid
This work was supported by Chinese National Key Technology R & D Program (2015BAD15B02), National Natural Science Foundation of China (31661143002, 31501034 and 31401421) and Yunnan Applied Basic Research Projects (2016FB060, 2016FA011).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Akpan U, Jimoh A, Mohammed A (2006) Extraction, characterization and modification of castor seed oil. Leonardo J Sci 8:43–52Google Scholar
- Baud S, Kelemen Z, Thévenin J, Boulard C, Blanchet S, To A et al (2016) Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by master transcriptional regulators in Arabidopsis seed. Plant Physiol 171:1099–1112. https://doi.org/10.1104/pp.16.00034 PubMedPubMedCentralGoogle Scholar
- Brown AP, Kroon JT, Swarbreck D, Febrer M, Larson TR, Graham IA, Caccamo M, Slabas AR (2012) Tissue-specific whole transcriptome sequencing in castor, directed at understanding triacylglycerol lipid biosynthetic pathways. PLoS One 7(2):e30100. https://doi.org/10.1371/journal.pone.0030100 CrossRefPubMedPubMedCentralGoogle Scholar
- Cao S, Kumimoto RW, Siriwardana CL, Risinger JR, Holt BF III (2011) Identification and characterization of NF-Y transcription factor families in the monocot model plant Brachypodium distachyon. PLoS One 6(6):e21805. https://doi.org/10.1371/journal.pone.0021805 CrossRefPubMedPubMedCentralGoogle Scholar
- Feng Z-J, He G-H, Zheng W-J, Lu P-P, Chen M, Gong Y-M, Ma Y-Z, Xu Z-S (2015) Foxtail millet NF-Y families: genome-wide survey and evolution analyses identified two functional genes important in abiotic stresses. Front Plant Sci 6:1142. https://doi.org/10.3389/fpls.2015.01142 PubMedPubMedCentralGoogle Scholar
- Lee DK, Kim HI, Jang G, Chung PJ, Jeong JS, Kim YS, Bang SW, Jung H, Choi YD, Kim JK (2015) The NF-YA transcription factor OsNF-YA7 confers drought stress tolerance of rice in an abscisic acid independent manner. Plant Sci 241:199–210. https://doi.org/10.1016/j.plantsci.2015.10.006 CrossRefPubMedGoogle Scholar
- Li W, Oono Y, Zhu J, He X, Wu J, Iida K, Lu XY, Cui X, Jin H, Zhu JK (2008) The Arabidopsis NF-YA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance. Plant Cell 20:2238–2251. https://doi.org/10.1105/tpc.108.059444 CrossRefPubMedPubMedCentralGoogle Scholar
- Myers ZA, Kumimoto RW, Siriwardana CL, Gayler KK, Risinger JR, Pezzetta D, Holt BF III (2016) NUCLEAR FACTOR Y, subunit C (NF-YC) transcription factors are positive regulators of photomorphogenesis in Arabidopsis thaliana. PLoS Gene 12(9):e1006333. https://doi.org/10.1371/journal.pgen.1006333 CrossRefGoogle Scholar
- Sato H, Mizoi J, Tanaka H, Maruyama K, Qin F, Osakabe Y, Morimoto K, Ohori T, Kusakabe K, Nagata M, Shinozaki K, Yamaguchi-Shinozaki K (2014) Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits. Plant Cell 26:4954–4973. https://doi.org/10.1105/tpc.114.132928 CrossRefPubMedPubMedCentralGoogle Scholar
- Siefers N, Dang KK, Kumimoto RW, Bynum WE IV, Tayrose G, Holt BF III (2009) Tissue-specific expression patterns of Arabidopsis NF-Y transcription factors suggest potential for extensive combinatorial complexity. Plant Physiol 149:625–641. https://doi.org/10.1104/pp.108.130591 CrossRefPubMedPubMedCentralGoogle Scholar
- Steidl S, Tüncher A, Goda H, Guder C, Papadopoulou N, Kobayashi T, Tsukagoshi N, Kato M, Brakhage AA (2004) A single subunit of a heterotrimeric CCAAT-binding complex carries a nuclear localization signal: piggyback transport of the pre-assembled complex to the nucleus. J Mol Biol 342:515–524CrossRefPubMedGoogle Scholar
- Tan H, Yang X, Zhang F, Zheng X, Qu C, Mu J, Fu F, Li J, Guan R, Zhang H, Wang G, Zuo J (2011) Enhanced seed oil production in canola by conditional expression of Brassica napus LEAFY COTYLEDON1 and LEC1-LIKE in developing seeds. Plant Physiol 156:1577–1588. https://doi.org/10.1104/pp.111.175000 CrossRefPubMedPubMedCentralGoogle Scholar
- Zhang T, Zhang D, Liu Y, Luo C, Zhou Y, Zhang L (2015b) Overexpression of a NF-YB3 transcription factor from Picea wilsonii confers tolerance to salinity and drought stress in transformed Arabidopsis thaliana. Plant Physiol Biochem 94:153–164. https://doi.org/10.1016/j.plaphy.2015.05.001 CrossRefPubMedGoogle Scholar