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Planta

, Volume 247, Issue 3, pp 559–572 | Cite as

Gene structure, expression pattern and interaction of Nuclear Factor-Y family in castor bean (Ricinus communis)

  • Yue Wang
  • Wei Xu
  • Zexi Chen
  • Bing Han
  • Mohammad E. Haque
  • Aizhong Liu
Original Article
  • 436 Downloads

Abstract

Main conclusion

Nuclear Factor-Y transcription factors, which function in regulating seed development (including storage reservoir accumulation) and responding to abiotic stresses, were identified and characterized in castor bean.

Nuclear Factor-Y (NF-Y) transcription factors in plants contain three subunits (NF-YA, NF-YB and NF-YC), and function as a heterodimer or heterotrimer complex in regulating plant growth, development and response to stresses. Castor bean (Ricinus communis, Euphorbiaceae) one of the most economically important non-edible oilseed crops, able to grow in diverse soil conditions and displays high tolerance to abiotic stresses. Due to increasing demands for its seed oils, it is necessary to elucidate the molecular mechanism underlying the regulation of growth and development. Based on the available genome data, we identified 25 RcNF-Y members including six RcNF-YAs, 12 RcNF-YBs and seven RcNF-YCs, and characterized their gene structures. Yeast two-hybrid assays confirmed the protein–protein interactions among three subunits. Using transcriptomic data from different tissues, we found that six members were highly or specifically expressed in endosperms (in particular, two LEC1-type members RcNF-YB2 and RcNF-YB12), implying their involvement in regulating seed development and storage reservoir accumulation. Further, we investigated the expression changes of RcNF-Y members in two-week-old seedlings under drought, cold, hot and salt stresses. We found that the expression levels of 20 RcNF-Y members tested were changed and three RcNF-Y members might function in response to abiotic stresses. This study is the first reported on genomic characterization of NF-Y transcription factors in the family Euphorbiaceae. Our results provide the basis for improved understanding of how NF-Y genes function in the regulation of seed development and responses to abiotic stresses in both castor bean and other plants in this family.

Keywords

Abiotic stress Castor bean Expression profiles NF-Y transcription factor Protein interaction 

Abbreviations

NF-Y

Nuclear Factor-F

Rc

Ricinus communis

LEC1

Leafy Cotyledon 1

CDS

Coding sequence

Y2H

Yeast two hybrid

Notes

Acknowledgements

This work was supported by Chinese National Key Technology R & D Program (2015BAD15B02), National Natural Science Foundation of China (31661143002, 31501034 and 31401421) and Yunnan Applied Basic Research Projects (2016FB060, 2016FA011).

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

425_2017_2809_MOESM1_ESM.pdf (986 kb)
Supplementary material 1 (PDF 986 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Yue Wang
    • 1
  • Wei Xu
    • 1
  • Zexi Chen
    • 1
    • 2
  • Bing Han
    • 1
    • 2
  • Mohammad E. Haque
    • 1
    • 2
  • Aizhong Liu
    • 1
  1. 1.Yunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of BotanyChinese Academy of SciencesKunmingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina

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