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Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites

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Abstract

Main conclusion

The method introduced here to grow F. hygrometrica in high concentrations of D 2 O is an excellent alternative to produce highly deuterated metabolites with broad applications in metabolic studies. Our mass spectrometry experiments strongly indicate the successful incorporation of deuterium into organic compounds.

Deuterated metabolites are useful tracers for metabolic studies, yet their wide utilization in research is limited by the multi-step total synthesis required to produce them in the laboratory. Alternatively, deuterated metabolites can be obtained from organisms grown in D2O or deuterated nutrients. This approach also has limitations as D2O in high concentrations negatively affects the survival of most organisms. Here we report the moss Funaria hygrometrica as an unusual high tolerant to D2O in liquid culture. We found that this moss is able to grow in up to 90% D2O, a condition lethal for many eukaryotes. Mass spectrometric analyses of F. hygrometrica extracts showed a strong deuteration pattern. The ability to tolerate high concentrations of D2O together with the development of a rich molecular toolbox makes F. hygrometrica an ideal system for the production of valuable deuterated metabolites.

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Acknowledgements

This study was supported in part by JSPS KAKENHI Grant number 25113010 to M.Y.H. The financial support was provided by CINVESTAV and CONACYT basic science grant FRONTERAS 2015-2/814 to R.W. The authors are grateful to Esperanza Ayala Gil, M.Sc., and Dr. David Gómez Zepeda for their support on mass spectrometry analysis.

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Authors and Affiliations

  1. Metabolic Systems Research Team, RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

    Fredd Vergara & Masami Hirai

  2. Plant Productivity Systems Research Group, RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Japan

    Misao Itouga

  3. National Laboratory of Genomics for Biodiversity, CINVESTAV Unidad Irapuato, Irapuato, Mexico

    Roberto Gamboa Becerra & José Juan Ordaz-Ortiz

  4. Department of Biotechnology and Biochemistry, CINVESTAV Unidad Irapuato, Irapuato, Mexico

    Robert Winkler

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  1. Fredd Vergara
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  2. Misao Itouga
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  3. Roberto Gamboa Becerra
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  4. Masami Hirai
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  5. José Juan Ordaz-Ortiz
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Correspondence to Fredd Vergara.

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Vergara, F., Itouga, M., Becerra, R.G. et al. Funaria hygrometrica Hedw. elevated tolerance to D2O: its use for the production of highly deuterated metabolites. Planta 247, 405–412 (2018). https://doi.org/10.1007/s00425-017-2794-5

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  • DOI: https://doi.org/10.1007/s00425-017-2794-5

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