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Holaphyllamine, a steroid, is able to induce defense responses in Arabidopsis thaliana and increases resistance against bacterial infection

  • Abderrakib Zahid
  • Rim Jaber
  • Ferdousse Laggoun
  • Arnaud Lehner
  • Isabelle Remy-Jouet
  • Olivier Pamlard
  • Sandra Beaupierre
  • Jérome Leprince
  • Marie-Laure Follet-Gueye
  • Maïté Vicré-Gibouin
  • Xavier Latour
  • Vincent Richard
  • Catherine Guillou
  • Patrice Lerouge
  • Azeddine Driouich
  • Jean-Claude Mollet
Original Article

Abstract

Main conclusion

A chemical screen of plant-derived compounds identified holaphyllamine, a steroid, able to trigger defense responses inArabidopsis thalianaand improve resistance against the pathogenic bacteriumPseudomonas syringaepv tomato DC3000.

A chemical screen of 1600 plant-derived compounds was conducted and allowed the identification of a steroid able to activate defense responses in A. thaliana at a concentration of 1 µM without altering growth. The identified compound is holaphyllamine (HPA) whose chemical structure is similar to steroid pregnanes of mammals. Our data show that HPA, which is not constitutively present in A. thaliana, is able to trigger the formation of reactive oxygen species, deposition of callose and expression of several pathogenesis-related genes of the salicylic and jasmonic acid pathways. In addition, the results show that pre-treatment of A. thaliana seedlings with HPA before infection with the pathogenic bacterium Pseudomonas syringae pv tomato DC3000 results in a significant reduction of symptoms (i.e., reduction of bacterial colonies). Using A. thaliana mutants, we have found that the activation of defense responses by HPA does not depend on BRI1/BAK1 receptor kinases. Finally, a structure/function study reveals that the minimal structure required for activity is a 5-pregnen-20-one steroid with an equatorial nucleophilic group in C-3. Together, these findings demonstrate that HPA can activate defense responses that lead to improved resistance against bacterial infection in A. thaliana.

Keywords

Callose Chemical screen Elicitor Holaphyllamine PATHOGENESIS-RELATED Plant defense Pseudomonas syringae pv tomato DC3000 ROS Steroid 

Abbreviations

BRI1/BAK1

BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR-LIKE KINASE1

dpi

Days post-inoculation

flg22

Flagellin22

HPA

Holaphyllamine

PR

Pathogenesis related

Pst

Pseudomonas syringae pv tomato DC3000

Supplementary material

425_2017_2755_MOESM1_ESM.pdf (1.9 mb)
Supplementary material 1 (PDF 1944 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Abderrakib Zahid
    • 1
    • 6
  • Rim Jaber
    • 1
  • Ferdousse Laggoun
    • 1
  • Arnaud Lehner
    • 1
  • Isabelle Remy-Jouet
    • 2
  • Olivier Pamlard
    • 3
  • Sandra Beaupierre
    • 3
  • Jérome Leprince
    • 4
  • Marie-Laure Follet-Gueye
    • 1
  • Maïté Vicré-Gibouin
    • 1
  • Xavier Latour
    • 5
  • Vincent Richard
    • 2
  • Catherine Guillou
    • 3
  • Patrice Lerouge
    • 1
  • Azeddine Driouich
    • 1
  • Jean-Claude Mollet
    • 1
    • 7
  1. 1.Normandie Univ, UniRouen, Laboratoire Glycobiologie et Matrice Extracellulaire végétale, SFR Normandie VégétalRouenFrance
  2. 2.Normandie Univ, UniRouen, Laboratoire Nouvelles Cibles Pharmacologiques du Traitement de la Dysfonction Endothéliale et de l’Insuffisance Cardiaque, INSERM, IRIBRouenFrance
  3. 3.Institut de Chimie des Substances Naturelles, CNRS, LabEx LERMITGif-sur-YvetteFrance
  4. 4.Normandie Univ, UniRouen, Laboratoire de Différenciation et Communication Neuronale et Neuroendocrine INSERM, IRIBRouenFrance
  5. 5.Normandie Univ, UniRouen, IUT Evreux, Laboratoire de Microbiologie Signaux et Microenvironnement, SFR Normandie VégétalRouenFrance
  6. 6.SATT Nord, GIS PhyNoPi CS80699CalaisFrance
  7. 7.Normandie Univ, UniRouen, Laboratoire Glycobiologie et Matrice Extracellulaire végétale (Glyco-MEV) EA4358Mont-Saint-AignanFrance

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