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Planta

, Volume 246, Issue 1, pp 91–103 | Cite as

An RNA-Seq transcriptome analysis revealing novel insights into aluminum tolerance and accumulation in tea plant

  • Yong Li
  • Jie Huang
  • Xiaowei Song
  • Ziwei Zhang
  • Ye Jiang
  • Yulu Zhu
  • Hua ZhaoEmail author
  • Dejiang Ni
Original Article

Abstract

Main conclusion

The tea plant ( Camellia sinensis L. O. Kuntze) is a high aluminum (Al) tolerant and accumulator species. Candidate genes related to Al tolerance in tea plants were assembled based on de novo transcriptome analysis. The homologs implied some common and distinct Al-tolerant mechanism between tea plants and rice, Arabidopsis and buckwheat.

In addition to high Al tolerance, the tea plant exhibits good performance exposure to a proper Al level, and accumulates high Al in the leaves without any toxicity symptom. Therefore, Al was considered as a hyperaccumulator and beneficial element for tea plants. However, the whole-genome molecular mechanisms accounting for Al-tolerance and accumulation remain unknown in tea plants. In this study, transcriptome analysis by RNA-Seq following a gradient Al-level exposure was assessed to further reveal candidate genes involved. Totally more than 468 million high-quality reads were generated and 213,699 unigenes were de novo assembled, among which 8922 unigenes were all annotated in the seven databases used. A large number of transporters, transcription factors, cytochrome P450, ubiquitin ligase, organic acid biosynthesis, heat shock proteins differentially expressed in response to high Al (P ≤ 0.05) were identified, which were most likely ideal candidates involved in the Al tolerance or accumulation. Furthermore, a few of the candidate Al-responsive genes related to Al sequestration, cell wall modification and organic acid excretion have been well elucidated as was already found in Arabidopsis, rice, and buckwheat. Thus, some consistent Al-tolerance mechanisms across the species are indicated. In conclusion, the transcriptome data provided useful insights of promising candidates for further characterizing the functions of genes involved in Al tolerance and accumulation in tea plants.

Keywords

Aluminum stress Candidate genes De novo transcriptome Camellia sinensis L.O. Kuntze 

Abbreviations

ALMT

Aluminum-activated malate transporter

ART1

Aluminum resistance transcription factor 1

DEGs

Differentially expressed genes

GST1

Glutathione-S transferase 1

MATE

Multidrug and toxic compound extrusion

STAR1, 2

Sensitive to aluminum rhizotoxicity 1, 2

STOP1

Sensitive to proton rhizotoxicity 1

Notes

Acknowledgements

This work was jointly supported by National Natural Science Foundation of China (31470406), the Fundamental Research Funds for the Central Universities (2662015BQ011, 2016BC001) and National Undergraduate Training Programs for Innovation and Entrepreneurship (201510504033).

Supplementary material

425_2017_2688_MOESM1_ESM.docx (794 kb)
Supplementary material 1 (DOCX 794 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Yong Li
    • 1
    • 2
  • Jie Huang
    • 1
    • 2
  • Xiaowei Song
    • 1
    • 2
  • Ziwei Zhang
    • 1
    • 2
  • Ye Jiang
    • 1
    • 2
  • Yulu Zhu
    • 1
    • 2
  • Hua Zhao
    • 1
    • 2
    Email author
  • Dejiang Ni
    • 1
    • 2
  1. 1.Key Laboratory of Horticultural Plant Biology of Ministry of EducationHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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