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Introduction of Thinopyrum intermedium ssp. trichophorum chromosomes to wheat by trigeneric hybridization involving Triticum, Secale and Thinopyrum genera

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Abstract

Main conclusion

Fluorescence in situ hybridization and molecular markers have confirmed that several chromosomes from Thinopyrum intermedium ssp. trichophorum have been added to a wheat background, which originated from a cross between a wheat– Thinopyrum partial amphiploid and triticale. The lines displayed blue grains and resistance to wheat stripe rust.

Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. With the aim to transfer novel genetic variation from Th. intermedium species for sustainable wheat breeding, a new trigeneric hybrid was produced by crossing an octoploid wheat–Th. intermedium ssp. trichophorum partial amphiploid with hexaploid triticale. Fluorescence in situ hybridization (FISH) revealed that Thinopyrum chromosomes were transmitted preferably and the number of rye chromosomes tended to decrease gradually in the selfed derivatives of the trigeneric hybrids. Four stable wheat–Th. intermedium chromosome substitution, addition and translocation lines were selected, and a 2JS addition line, two substitution lines of 4JS(4B) and 4J(4B), and a small 4J.4B translocation line were identified by FISH and molecular markers. It was revealed that the gene(s) responsible for blue grains may located on the FL0.60–1.00 of long arm of Th. intermedium-derived 4J chromosome. Disease resistance screenings indicated that chromosomes 4JS and 2JS appear to enhance the resistance to stripe rust in the adult plant stage. The new germplasm with Th. intermedium introgression shows promise for utilization of Thinopyrum chromosome segments in future wheat improvement.

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Acknowledgements

We gratefully thank Drs I. Dundas at the University of Adelaide, and Dr. Peng Zhang at University of Sydney, Australia for reviewing the manuscript, and to the support of The National Key Research and Development Program of China (2016YFD0102000), Basic and Applied Project from Department of Science and Technology, Sichuan (2016JY0075), and National Natural Science Foundation of China (31171542).

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Authors and Affiliations

  1. Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China

    Jianbo Li, Tao Lang, Bin Li, Zhihui Yu, Hongjin Wang, Guangrong Li & Zujun Yang

  2. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China

    Ennian Yang

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  1. Jianbo Li
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  2. Tao Lang
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  3. Bin Li
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Correspondence to Zujun Yang.

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425_2017_2669_MOESM1_ESM.jpg

Supplementary material 1 Chromosome comparison of 4JS from X24C5 and 4JS-St from X482. Arrow shows the putative position for the St-JS translocation breakpoint. Left showed the signals of Oligo-pSc119.2 (green) + Oligo-pTa535 (red), middle showed the signals of pDb12H (green), and right showed the signals of Oligo-pSt122 (green) (JPEG 39 kb)

425_2017_2669_MOESM2_ESM.jpg

Supplementary material 2 FISH patterns of Th. intermedium chromosomes in Z2 and X24C14. Arrow indicates the possible position of the breakpoint. Left chromosomes showed the signals of pDb12H (green), middle showed the signals of Oligo-pSc119.2 (green) + Oligo-pTa535 (red), and right indicated the signals of Oligo-pSt122 (green) (JPEG 31 kb)

Supplementary material 3 (DOC 94 kb)

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Li, J., Lang, T., Li, B. et al. Introduction of Thinopyrum intermedium ssp. trichophorum chromosomes to wheat by trigeneric hybridization involving Triticum, Secale and Thinopyrum genera. Planta 245, 1121–1135 (2017). https://doi.org/10.1007/s00425-017-2669-9

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