Synergistic effects between [Si-hemicellulose matrix] ligands and Zn ions in inhibiting Cd ion uptake in rice (Oryza sativa) cells
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Our study demonstrated that Zn alleviated Cd toxicity in the presence of Si in the cell walls by Zn 2+ binding to ligands through the formation of the [Si-hemicellulose matrix]Zn complexes that restrict the uptake of Cd.
The plant cell wall exhibits preferential sites for the accumulation of metals at toxic concentrations. Through modification of wall polysaccharide components, elements, such as silicon (Si) and zinc (Zn), may play active roles in alleviating the toxicity of heavy metals, including cadmium (Cd). However, enhanced tolerance for Cd stress may rely on synergistic effects between nutrient elements. Here, we cultured Si-accumulating suspension cells of rice (Oryza sativa) exposed to Cd and Zn treatments, either separately or in combination, and investigated cells using noninvasive microtest technology (NMT), inductively coupled plasma mass spectroscopy (ICP-MS) and atomic force microscopy (AFM). We found that Zn alleviated Cd toxicity in the presence of Si in the cell walls by binding of Zn2+ to ligands through the formation of the [Si-hemicellulose matrix]Zn complexes and co-precipitates to greatly inhibit Cd2+ uptake into cells. This, in turn, induced the lower expression of Cd-related transporters. This synergistic effect could be decisive for the survival of cells under conditions of high Cd concentrations.
KeywordsCadmium (Cd) Zinc (Zn) Silicon (Si) [Si-hemicellulose matrix] ligands Synergistic effects Cell wall Rice (Oryza sativa) single cell
This work was supported by the National Natural Science Foundation of China (31672222 and 31172027) and the Fundamental Research Funds for the Central Universities (2662015PY206).
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Conflict of interest
The authors declare that they have no conflict of interest.
- Ahmed M, Kamran A, Asif M, Qadeer U, Ahmed ZI, Goyal A (2013) Silicon priming: a potential source to impart abiotic stress tolerance in wheat: a review. Aust J Crop Sci 7:484Google Scholar
- Ghareeb H, Bozsó Z, Ott PG, Repenning C, Stahl F, Wydra K (2011) Transcriptome of silicon-induced resistance against Ralstonia solanacearum in the silicon non-accumulator tomato implicates priming effect. Physiol Mol Plant P 75:83–89Google Scholar
- Kumari M, Sinhal VK, Srivastava A, Singh VP (2011) Zinc alleviates cadmium induced toxicity in Vigna radiata (L.) Wilczek. J Phytol 3:43–46Google Scholar
- Szuster-Ciesielska A, Stachura A, Słotwińska M, Kamińska T, Śnieżko R, Paduch R, Abramczyk D, Filar J, Kandefer-Szerszeń M (2000) The inhibitory effect of zinc on cadmium-induced cell apoptosis and reactive oxygen species (ROS) production in cell cultures. Toxicology 145:159–171CrossRefPubMedGoogle Scholar