Planta

, Volume 245, Issue 3, pp 611–622

Naturally occurring high oleic acid cottonseed oil: identification and functional analysis of a mutant allele of Gossypium barbadense fatty acid desaturase-2

  • Jay Shockey
  • Michael Dowd
  • Brian Mack
  • Matthew Gilbert
  • Brian Scheffler
  • Linda Ballard
  • James Frelichowski
  • Catherine Mason
Original Article

Abstract

Main conclusion

Some naturally occurring cotton accessions contain commercially attractive seed oil fatty acid profiles. The likely causal factor for a high-oleate trait in pima cotton (Gossypium barbadense) accession GB-713 is described here.

Vegetable oils are broadly used in the manufacture of many human and animal nutritional products, and in various industrial applications. Along with other well-known edible plant oils from soybean, corn, and canola, cottonseed oil is a valuable commodity. Cottonseed oil is a co-product derived from the processing of cottonseed fiber. In the past, it was used extensively in a variety of food applications. However, cottonseed oil has lost market share in recent years due to less than optimal ratios of the constituent fatty acids found in either traditional or partially hydrogenated oil. Increased awareness of the negative health consequences of dietary trans-fats, along with the public wariness associated with genetically modified organisms has created high demand for naturally occurring oil with high monounsaturate/polyunsaturate ratios. Here, we report the discovery of multiple exotic accessions of pima cotton that contain elevated seed oil oleate content. The genome of one such accession was sequenced, and a mutant candidate fatty acid desaturase-2 (FAD2-1D) gene was identified. The mutant protein produced significantly less linoleic acid in infiltrated Arabidopsis leaf assays, compared to a repaired version of the same enzyme. Identification of this gene provides a valuable resource. Development of markers associated with this mutant locus will be very useful in efforts to breed the high-oleate trait into agronomic fiber accessions of upland cotton.

Keywords

Cottonseed Fatty acid desaturase Oleic acid Linoleic acid 

Abbreviations

CTAB

Cetyltrimethylammonium bromide

DNA

Deoxyribonucleic acid

FAD

Fatty acid desaturase

FAME

Fatty acid methyl ester

FID

Flame ionization detection

GC

Gas chromatography

GRAS

Generally recognized as safe

GRIN

Germplasm Resources Information Network

NCGC

U.S. National Cotton Germplasm Collection

PCR

Polymerase chain reaction

PHO

Partially hydrogenated oil

RT

Reverse transcription

Supplementary material

425_2016_2633_MOESM1_ESM.docx (44 kb)
Supplementary material 1 (DOCX 44 kb)
425_2016_2633_MOESM2_ESM.xlsx (25 kb)
Supplementary material 2 (XLSX 24 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg (outside the USA) 2016

Authors and Affiliations

  1. 1.Commodity Utilization Research Unit, United States Department of Agriculture-Agricultural Research ServiceSouthern Regional Research CenterNew OrleansUSA
  2. 2.Food and Feed Safety Research Unit, United States Department of Agriculture-Agricultural Research ServiceSouthern Regional Research CenterNew OrleansUSA
  3. 3.Genomics and Bioinformatics Research UnitUnited States Department of Agriculture-Agricultural Research ServiceStonevilleUSA
  4. 4.Crop Germplasm Research UnitUnited States Department of Agriculture-Agricultural Research ServiceCollege StationUSA

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