, Volume 244, Issue 6, pp 1303–1313 | Cite as

Does a voltage-sensitive outer envelope transport mechanism contributes to the chloroplast iron uptake?

  • Ádám Solti
  • Krisztina Kovács
  • Brigitta Müller
  • Saúl Vázquez
  • Éva Hamar
  • Hong Diep Pham
  • Brigitta Tóth
  • Javier Abadía
  • Ferenc Fodor
Original Article


Main conclusion

Based on the effects of inorganic salts on chloroplast Fe uptake, the presence of a voltage-dependent step is proposed to play a role in Fe uptake through the outer envelope.

Although iron (Fe) plays a crucial role in chloroplast physiology, only few pieces of information are available on the mechanisms of chloroplast Fe acquisition. Here, the effect of inorganic salts on the Fe uptake of intact chloroplasts was tested, assessing Fe and transition metal uptake using bathophenantroline-based spectrophotometric detection and plasma emission-coupled mass spectrometry, respectively. The microenvironment of Fe was studied by Mössbauer spectroscopy. Transition metal cations (Cd2+, Zn2+, and Mn2+) enhanced, whereas oxoanions (NO3 , SO4 2−, and BO3 3−) reduced the chloroplast Fe uptake. The effect was insensitive to diuron (DCMU), an inhibitor of chloroplast inner envelope-associated Fe uptake. The inorganic salts affected neither Fe forms in the uptake assay buffer nor those incorporated into the chloroplasts. The significantly lower Zn and Mn uptake compared to that of Fe indicates that different mechanisms/transporters are involved in their acquisition. The enhancing effect of transition metals on chloroplast Fe uptake is likely related to outer envelope-associated processes, since divalent metal cations are known to inhibit Fe2+ transport across the inner envelope. Thus, a voltage-dependent step is proposed to play a role in Fe uptake through the chloroplast outer envelope on the basis of the contrasting effects of transition metal cations and oxoaninons.


Chloroplast Envelope membrane Iron metabolism Mössbauer spectrosopy Voltage-dependent transport 



Light Harvesting Complex II apoprotein


Carbonyl cyanide m-chlorophenyl-hydrazone




Transmembrane electrochemical potential


Inner envelope


Outer envelope


Rubisco large subunit



This work was supported by the Grants financed by the National Office for Research, Development, and Innovation, Hungary (OTKA-NKFIH PD-112047 and PD-111979), the Spanish Ministry of Economy and Competitivity (Project AGL2013–42175-R, co-financed with FEDER) and the Aragón Government (Group A03) to J.A. Á.S. was also supported by the Bolyai János Research Scholarship of the Hungarian Academy of Sciences (BO/00207/15/4).

Supplementary material

425_2016_2586_MOESM1_ESM.docx (2 mb)
Supplementary material 1 (DOCX 2069 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Ádám Solti
    • 1
  • Krisztina Kovács
    • 2
  • Brigitta Müller
    • 1
  • Saúl Vázquez
    • 3
    • 5
  • Éva Hamar
    • 1
  • Hong Diep Pham
    • 1
  • Brigitta Tóth
    • 4
  • Javier Abadía
    • 3
  • Ferenc Fodor
    • 1
  1. 1.Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, Faculty of SciencesEötvös Loránd UniversityBudapestHungary
  2. 2.Laboratory of Nuclear Chemistry, Department of Analytical Chemistry, Institute of Chemistry, Faculty of SciencesEötvös Loránd UniversityBudapestHungary
  3. 3.Department of Plant Nutrition, Aula Dei Experimental StationSpanish Council for Scientific Research (CSIC)SaragossaSpain
  4. 4.Department of Agricultural Botany, Crop Physiology and Biotechnology, Institute of Crop Sciences, Faculty of Agricultural and Food Sciences and Environmental ManagementUniversity of DebrecenDebrecenHungary
  5. 5.Faculty of Science, School of BiosciencesUniversity of NottinghamLeicestershireUK

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