Understanding plant cell-wall remodelling during the symbiotic interaction between Tuber melanosporum and Corylus avellana using a carbohydrate microarray
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A combined approach, using a carbohydrate microarray as a support for genomic data, has revealed subtle plant cell-wall remodelling during Tuber melanosporum and Corylus avellana interaction.
Cell walls are involved, to a great extent, in mediating plant–microbe interactions. An important feature of these interactions concerns changes in the cell-wall composition during interaction with other organisms. In ectomycorrhizae, plant and fungal cell walls come into direct contact, and represent the interface between the two partners. However, very little information is available on the re-arrangement that could occur within the plant and fungal cell walls during ectomycorrhizal symbiosis. Taking advantage of the Comprehensive Microarray Polymer Profiling (CoMPP) technology, the current study has had the aim of monitoring the changes that take place in the plant cell wall in Corylus avellana roots during colonization by the ascomycetous ectomycorrhizal fungus T. melanosporum. Additionally, genes encoding putative plant cell-wall degrading enzymes (PCWDEs) have been identified in the T. melanosporum genome, and RT-qPCRs have been performed to verify the expression of selected genes in fully developed C. avellana/T. melanosporum ectomycorrhizae. A localized degradation of pectin seems to occur during fungal colonization, in agreement with the growth of the ectomycorrhizal fungus through the middle lamella and with the fungal gene expression of genes acting on these polysaccharides.
KeywordsCarbohydrate-Active enZYmes CoMPP Ectomycorrhiza Hazel Plant cell wall Tuber
Comprehensive microarray polymer profiling
Plant cell-wall degrading enzymes
The Authors would like to thank Marta Vallino (Institute for Sustainable Plant Protection, Turin) for her support in the TEM observations and Annegret Kohler (INRA, Nancy) for her work on the development and management of the T. melanosporum transcriptome datasets. F. Martin’s laboratory is funded and supported by the French National Research Agency through the Laboratory of Excellence ARBRE (Grant no. ANR-11-LBX-002-01) and the Plant–Microbe Interactions Project, Genomic Science Programme, of the US Department of Energy, Office of Science, Biological, and Environmental Research (Grant No. DE-AC05-00OR22725).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Benedetti M, Pontiggia D, Raggi S, Cheng Z, Scaloni F, Ferrari S, Ausubel FM, Cervone F, De Lorenzo G (2015) Plant immunity triggered by engineered in vivo release of oligogalacturonides, damage-associated molecular patterns. Proc Natl Acad Sci USA 112:5533–5538CrossRefPubMedPubMedCentralGoogle Scholar
- Hacquard S, Tisserant E, Brun A, Legué V, Martin F, Kohler A (2013) Laser microdissection and microarray analysis of Tuber melanosporum ectomycorrhizas reveal functional heterogeneity between mantle and Hartig net compartments. Environ Microbiol 15:1853–1869. doi: 10.1111/1462-2920.12080 CrossRefPubMedGoogle Scholar
- Johnsen HR, Striberny B, Olsen S, Vidal-Melgosa S, Fangel JU, Willats WGT, Rose JKC, Krause K (2015) Cell wall composition profiling of parasitic giant dodder (Cuscuta reflexa) and its hosts: a priori differences and induced changes. New Phytol 207:805–816. doi: 10.1111/nph.13378 CrossRefPubMedGoogle Scholar
- Le Tacon F, Zeller B, Plain C, Hossann C, Bréchet C, Martin F, Kohler A, Villerd J, Robin C (2015) Study of nitrogen and carbon transfer from soil organic matter to Tuber melanosporum mycorrhizas and ascocarps using 15N and 13C soil labelling and whole-genome oligoarrays. Plant Soil 395:351–373CrossRefGoogle Scholar
- Lionetti V, Francoccia F, Ferrari S, Volpi C, Bellincampi D, Galletti R, D’Ovidio R, De Lorenzo G, Cervone F (2009) Engineering the cell wall by reducing de-methyl-esterified homogalacturonan improves saccharification of plant tissues for bioconversion. Proc Natl Acad Sci USA 107:616–621CrossRefPubMedPubMedCentralGoogle Scholar
- Lodish H, Berk A, Zipursky SL et al (2000) Molecular cell biology, 4th edn. Section 22.5: The dynamic plant cell wall. WH Freeman, New York. http://www.ncbi.nlm.nih.gov/books/NBK21709/
- Moller I, Marcus SE, Haeger A, Verhertbruggen Y, Verhoef R, Schols H, Ulvskov P, Mikkelsen JD, Knox JP, Willats W (2008) High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray profiles. Glycoconj J 25:37–48CrossRefPubMedGoogle Scholar
- Montanini B, Levati E, Bolchi A, Kohler A, Morin E, Tisserant E, Martin F, Ottonello S (2011) Genome-wide search and functional identification of transcription factors in the mycorrhizal fungus Tuber melanosporum. New Phytol 189:736–750. doi: 10.1111/j.1469-8137.2010.03525.x CrossRefPubMedGoogle Scholar
- Nagendran S, Hallen-Adams HE, Paper JM, Aslama N, Walton JD (2009) Reduced genomic potential for secreted plant cell-wall degrading enzymes in the ectomycorrhizal fungus Amanita bisporigera, based on the secretome of Trichoderma reesei. Fungal Genet Biol 46:427–435. doi: 10.1016/j.fgb.2009.02.001 CrossRefPubMedGoogle Scholar
- Pedersen HL, Fangel JU, McCleary B, Ruzanski C, GroRydahl MG, Ralet M-C, Farkas V, Schantz L, Marcus SE, Andersen MCF, Field R, Ohlin M, Knox JP, Clausen MH, Willats WGT (2012) Versatile high resolution oligosaccharide microarrays for plant glycobiology and cell wall research. J Biol Chem 287:39429–39438CrossRefPubMedPubMedCentralGoogle Scholar
- Sillo F, Zampieri E, Giordano L, Lione G, Colpaert JV, Balestrini R, Gonthier P (2015) Identification of genes differentially expressed during the interaction between the plant symbiont Suillus luteus and two plant pathogenic allopatric Heterobasidion species. Mycol Progress 14:106CrossRefGoogle Scholar
- Veneault-Fourrey C, Commun C, Kohler A, Morin E, Balestrini R, Plett J, Danchin E, Coutinho P, Wiebenga A, de Vries RP, Henrissat B, Martin F (2014) Genomic and transcriptomic analysis of Laccaria bicolor CAZome reveals insights into polysaccharides remodelling during symbiosis establishment. Fungal Biol Gen 72:168–181CrossRefGoogle Scholar