, Volume 242, Issue 3, pp 721–732 | Cite as

Comparative analysis of antioxidant, antimicrobiological and cytotoxic activities of native and fermented chamomile ligulate flower extracts

  • Aleksandra CvetanovićEmail author
  • Jaroslava Švarc-Gajić
  • Zoran Zeković
  • Saša Savić
  • Jelena Vulić
  • Pavle Mašković
  • Gordana Ćetković
Original Article


Main conclusion

The work investigated differences in apigenin content, as well as in other compounds, and examined the chemical profiles, antioxidant, antimicrobial and cytotoxic effects of extracts obtained from native and fermented chamomile ligulate flowers.

Chamomile (Chamomilla recutita L.) has a long history of being used as a medicinal plant due to many health benefits, including antiinflammatory, anticancer, antispasmodic, radical-scavenging effects and others. Apigenin is recognized as one of the most bioactive phenolic compounds in chamomile. In comparison to its bound forms, which include mostly apigenin-7-O-β-glucoside and various acylated forms, the aglycone is attributed with much higher bioactivity. Due to this fact, in this work ligulate florets of chamomile anthodium were subjected to a fermentation process using native chamomile enzymes to hydrolyze bound forms of apigenin to free aglycone. The contents of apigenin and apigenin-7-O-β-glucoside were determined in both fermented and nonfermented samples by UHPLC-MS–MS analysis to define the efficiency of conversion. After defining their chemical profiles, the extracts of fermented and nonfermented chamomile samples were also compared with respect to their antioxidant, antimicrobial and cytotoxic effects. The antioxidant effects of the obtained extracts were defined by electron spin resonance analysis for hydroxyl and superoxide radicals. The antimicrobial activity was defined for eight microbial strains, whereas cytotoxic activity was evaluated using two human cell lines (human cervix carcinoma and human rhabdomyosarcoma) and murine fibroblasts.


Chamomile Polyphenols ESR spectroscopy Cytotoxic activity Antimicrobial activity 



The present work was carried out within the project of the Serbian Ministry of Education, Science and Technological Development (Project No. TR 31013). The authors are grateful to dr Dušan Adamović, Institute of Field and Vegetable Crops, Bački Petrovac, Serbia, for his support in supplying plant material.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Aleksandra Cvetanović
    • 1
    Email author
  • Jaroslava Švarc-Gajić
    • 1
  • Zoran Zeković
    • 1
  • Saša Savić
    • 2
  • Jelena Vulić
    • 1
  • Pavle Mašković
    • 3
  • Gordana Ćetković
    • 1
  1. 1.Department of Biotechnology and Pharmaceutical Engineering, Faculty of TechnologyUniversity of Novi SadNovi SadSerbia
  2. 2.Faculty of TechnologyLeskovacSerbia
  3. 3.Faculty of AgricultureČačakSerbia

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